Simultaneous Determination of Three Alkaloids in Extracts from Gelsemium elegans Benth. by HPLC

Abstract

Abstract: Objective To develop a method for the determination of three alkaloids in extracts from Gelsemium elegans Benth. by HPLC. Methods YMC C₁₈ (250 mm × 4. 6 mm, 5 μm) column was used with acetonitriles-0.025 mol·L^-1 KH₂PO₄ solution (phosphoric acid was used to adjust pH to 3.0) with gradient elution as the mobile phase and 1.0 mL·min^-1 as the flow rate. The detection wavelength was 254 nm. Results The linear ranges of koumine, gelsemine and 16-epivoacarpine were 32.2~322, 34.4~344, 6.2~62 μg·mL^-1, respectively. The average recoveries were 98.6%, 101.1%, 98.3% with RSD of 1.7%, 1.3%, 1.1%, respectively. Conclusion This method is accurate, simple and reliable, and can be used for quality control of extracts from Gelsemium elegans Benth.

Key words: Gelsemium elegans Benth, koumine, gelsemine, 16-epivoacarpine, quantitative determination

CLC Number:

R917

WANG Lin, SUN Lin, LIU Hui-ying, ZHAO Nan, MENG Fan-hao. Simultaneous Determination of Three Alkaloids in Extracts from Gelsemium elegans Benth. by HPLC[J]. Chinese Journal of Pharmacovigilance, 2017, 14(3): 161-164.

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