Chinese Journal of Pharmacovigilance ›› 2024, Vol. 21 ›› Issue (12): 1363-1370.
DOI: 10.19803/j.1672-8629.20240559

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Digital Characterization of Surface Characteristics of Polygonum multiflorum Thunb. Pieces and Correlations with Chemical Composition Contents

YANG Yanrong1, SHI Jia2△, LI Yanyi3, WEI Feng2, MA Shuangcheng4, WANG Fang1, WANG Qi2,*   

  1. 1Shenyang Pharmaceutical University, School of Functional Food and Wine, Shenyang 110016, China;
    2National Institutes for Food and Drug Control, Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, Beijing 100050, China;
    3School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;
    4Chinese Pharmacopoeia Commission, Beijing 100061, China;
    5State Key Laboratory of Drug Regulatorg Science, Beijing 100061, China
  • Received:2024-08-12 Online:2024-12-15 Published:2024-12-16

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Abstract: Objects To establish a method for evaluating the quality of Polygonum multiflorum Thunb. pieces based on the surface characteristic chromaticity value, that is, the surface color of Polygonum multiflorum Thunb. Methods The surface features of different parts of Polygonum multiflorum Thunb. were collected under a stereo fluorescence microscope, and the chromaticity values of R, G, B, L*, a* and b* were obtained with photo shop cs6 software. The contents of stilbene glucoside, emodin, emodin -8-O-β-D- glucoside, emodin methyl ether and emodin methyl ether -8-O-β-D- glucoside in Polygonum multiflorum Thunb. were determined by UPLC-QQ-MS/MS, and the correlations between the chromaticity value of Polygonum multiflorum Thunb. and the contents of the five components were analyzed via cluster analysis (HCA), one-way ANOVA and Pearson correlation analysis. Results The results of HCA showed that Polygonum multiflorum Thunb. pieces from different habitats were grouped into three categories, which were light yellow-white, yellow-brown and reddish-brown, indicating that the colors of Polygonum multiflorum Thunb. pieces from different habitats varied. The results of one-way ANOVA showed that there were significant differences in the contents of stilbene glycoside and emodin in Polygonum multiflorum Thunb. pieces with different cross sections. Macroscopically, the stilbene glycoside content was higher in Polygonum multiflorum Thunb. pieces with light yellow and white cross sections, emodin content was higher in those with yellow-brown cross sections, and the content of stilbene glycoside and emodin was lower in those with red-brown cross sections. The results of correlation analysis showed that there was also a correlation between the chromaticity value and the related content of Polygonum multiflorum Thunb. The skin chromaticity value A of Polygonum multiflorum Thunb. was negatively correlated with the content of emodin methyl ether. Conclusion The established method is accurate and user-friendly. The quantification of chromaticity values can provide data for empirical identification of Polygonum multiflorum Thunb. It is a compelling interpretation of the traditional identification experience related to Polygonum multiflorum Thunb. and an combination of traditional experience and modern science to reveal the correlations between the cross-section color of decoction pieces and related chemical components of Polygonum multiflorum Thunb.

Key words: Polygonum multiflorum Thunb., UPLC-QQQ-MS/MS, Stilbene Glycosides, Flavonoids, Character, Chrominance, Chemical Composition, Digitization

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