Simultaneous determination of fifteen components in Xuanfudaizhe decoction by UPLC-MS/MS

doi:10.19803/j.1672-8629.20220735

Abstract

Abstract: Objective To establish a method for simultaneous determination of fifteen components in Xuanfudaizhe decoction by UPLC-MS/MS. Methods The separation was performed on a Shim-pack GIST C₁₈column (2.1 mm × 100 mm, 2.0 μm) with acetonitrile-0.1% formic acid as the mobile phase under gradient elution at a flow rate of 0.3 mL·min^-1. The column temperature was 35℃. The detection was carried out via electrospray ionization (ESI). Multiple-reaction monitoring (MRM) scanning was used for quantification in a negative ion mode. Results Liquiritin, glycyrrhizic acid, liquiritigenin, isoliquiritigeni, isoliquiritin, isoliquiritin apioside, neoliquiritin, chlorogenic acid, cryptochlorogenic acid, ferulic acid, isochlorogenic acid A, isochlorogenic acid C, ginsenoside Rb₁, ginsenoside Re and 6-gingerol had good linear relationships in their respective concentration ranges (r>0.998 6). The average recovery ranged from 97.0% to 103.1%, and the precision, stability and repeatability of the method were good. Conclusion This method is simple and accurate, which can provide reference for Xuanfudaizhe decoction and its preparations.

Key words: UPLC-MS/MS, Xuanfudaizhe decoction, flavonoids, organic acids, saponins, content determination

CLC Number:

R927.1

GAO Le, WANG Xiang, MA Jingmei, ZHANG Zezhao, WANG Xinguo, NIU Liying. Simultaneous determination of fifteen components in Xuanfudaizhe decoction by UPLC-MS/MS[J]. Chinese Journal of Pharmacovigilance, 2023, 20(9): 987-991.

References

[1] The directory of the first group of the classic formulas issued by the first batch of classic formula directory[J]. Jiangsu Journal of Traditional Chinese Medicine(江苏中医药), 2018(7):1-10.
[2] LI SS, HOU YJ, ZHANG L, et al.Historical evolution of Xuanfu Daizhe Decoction[J]. China Journal of Chinese Materia Medic(中国中药杂志), 2022, 47(15): 4033-4041.
[3] BAI Y, CHENG CH, CAO S.Research progress of Xuanfu Daizhe decoction in treatment of digestive diseases[J]. Acta Chinese Medicine(中医学报), 2022, 37(5): 997-1004.
[4] TIAN S, LIU WL, SU KH, et al.Progress in clinical application of Xuanfu Daizhe decoction[J]. Asia-Pacific Traditional Medicine(亚太传统医药), 2021, 17(7): 205-209.
[5] ZHANG JZ, LIU F, ZHANG Y, et al.Impacts of water volume and decoction time on the dissolubility of five effective components in Xuanfu Daizhe Tang prepared with tisane machine[J]. World Journal of Integrated Traditional and Western Medicine(世界中西医结合杂志), 2013, 8(5): 457-460.
[6] ZHANG JC, LIU F, ZHANG Y, et al.Immersion time influence on 5 major bioactive components of Xuanfu Daizhe Decoction decocted by modern decocting medicinal herbs machine[J]. Global Traditional Chinese Medicine(环球中医药), 2013, 6(2): 88-91.
[7] WANG B, QIN KM, YAO ZQ, et al.Orthogonal design for optimizing decoction process of Xuanfu Daizhe Decoction with multi-targets[J]. Chinese Traditional Patent Medicine(中成药), 2012, 34(11): 2106-2110.
[8] XIE H, LI L, LI HH, et al.Characteristic chromatogram and index components content of substance benchmark of Xuanfu Daizhe Decoction[J]. China Journal of Chinese Materia Medic(中国中药杂志), 2022, 47(8): 2090-2098.
[9] ZHU MZ, CHEN GL, WU JL, et al.Recent development in mass spectrometry and its hyphenated techniques for the analysis of medicinal plants[J]. Phytochem Analysis:PCA, 2018, 29(4): 365.
[10] LI YY, WANG Y, ZHANG NP, et al.Effects of different harvest seasons on anthraquinone contents of Polygoni Multiflori Radix based on UPLC-MS/MS[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2022, 19(12): 1277-1284.
[11] LI YY, WANG Y, ZHANG NP, et al.Effects of duration of steaming on 26 chemical compositions of Polygoni Multiflori Radix based on UPLC-MS/MS detection technology[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2022, 19(12): 1295-1302.
[12] FU CY, WANG HY, LIU YH, et al.Simultaneous determination of eight components in Xuefu Zhuyu Decoction based on UPLC-ESI-IT-TOF-MS method[J]. Nat Prod Res Dev(天然产物研究与开发), 2020, 32(6): 1006.
[13] SHI JX, SHI XG.A Preliminary study on the conversion and usage of classical famous prescriptions[J]. Jiangxi Journal of Traditional Chinese Medicine(江西中医药), 2016, 47(11): 18-20.
[14] HUANG YJ.Study on drug dosage and related problems in Shang Han Lun[D]. Beijing University Of Chinese Medicine(北京中医药大学), 2007.
[15] MENG CQ, WANG RP.Discussion on the dosage and reasonable prescription in 《Shang Han Lun》[J]. Journal of Practical Traditional Chinese Medicine(实用中医药杂志), 2016, 32(8): 838-839.
[16] KE XF, ZHAO ZZ, ZHANG YP, et al.The problem of drug dosage in“Shang Han Lun”and“Jin Gui Yao Lue”[J]. Shanghai Journal of Traditional Chinese Medicine(上海中医药杂志), 1983(12): 36-38.
[17] ZHANG ZH, CHU GS, WANG HY, et al.Simultaneous determination of five phenolic acids in Xanthii Fructus by QAMS[J]. Chinese Journal of Pharmaceutical Analysis(药物分析杂志), 2021, 41(9): 1612-1620.
[18] ZHOU YM, FENG C, SHI YH, et al.Study on fingerprint of licorice extract and the content determination of its multi-indicator[J].Chinese Journal of Modern Applied Pharmacy(中国现代应用药学), 2020, 37(9): 1046-1051.