Abstract: Objective To screen the best extraction technology of fufangchaihugegen granules by evaluating the transfer rate of active constituent, and anti-inflammatory analgesic activity. Methods HPLC method was used to investigate the transfer rate of baicalin, puerarin, and forsythin under different extraction processes. The ear swelling rate of mice and acetic acid-induced writhing test in mice were used to investigate the anti-inflammatory analgesic activity of fufangchaihugegen granules under different extraction processes. Results The best extraction technology is NO.4. The volatile oil of notopterygium, bupleuri, and forsythia was extracted by steam distillation method. The residue was decocted three times, combined with isatis root and platycodon grandiflorum to obtain liquid extract. Then puerarin and baicalin were extracted by 60% alcohol, and concentrated it as liquid extract by alcohol. The two-part flow extract was combined and the volatile oil was added. Conclusion The extraction process of fufangchaihugegen granules screened by the active component transfer rate experiment and the anti-inflammatory analgesic activity is scientific, reasonable, and feasible. It is suitable for the research and development of the traditional Chinese medicine preparation.

Key words: fufangchaihugegen particles, extraction technology, transferring rate, anti-inflammatory and analgesic effects