Quantitative Characterization and Chemometric Discrimination of 24 Coumarin Constituents in Peucedanum Medicinal Materials

doi:10.19803/j.1672-8629.20250213

Abstract

Abstract: Objective To establish a multi-component simultaneous quantitative analytical method for 24 coumarin constituents in Peucedanum medicinal materials, elucidate the differences in chemical properties and species-specific markers between Peucedanum praeruptorum Dunn (Qianhu) and Peucedanum decursivum (Miq.) Maxim. (Zihua Qianhu) in order to provide a reference for varietal identification and for evaluation of geographical authenticity. Methods Using UPLC-MS/MS technology, a multi-component quantitative analytical method was established, which was validated in terms of linearity, sensitivity, precision, stability, matrix effects, and recovery rates. Chemometric techniques, including principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and hierarchical clustering analysis (HCA), were applied to profile the chemical patterns of multi-batch samples from Peucedanum praeruptorum Dunn and Peucedanum decursivum (Miq.) Maxim. Results The analytical method for 24 coumarins in the genus Peucedanum established based on the UPLC-MS/MS method passed the methodological validation, and all the investigated items met the analytical requirements. The analysis of contents of the components in Peucedanum praeruptorum Dunn and Peucedanum decursivum (Miq.) Maxim. showed that there were significant species-specific and chemical characteristic differences in the distribution of coumarin components between these two plants. The contents of such compounds as nodakenin (10-30 times) and 7-hydroxycoumarin (2-10 times) in Peucedanum praeruptorum Dunn were much lower than those of Peucedanum decursivum (Miq.) Maxim. PCA, PLS-DA, and HCA indicated that the 24 coumarin components were distinctly separated in the chemical space between Peucedanum praeruptorum Dunn and Peucedanum decursivum (Miq.) Maxim. The core markers of Peucedanum praeruptorum Dunn were praeruptorin A and praeruptorin B in linear furanocoumarins, while Peucedanum decursivum (Miq.) Maxim. was rich in isodecursinol and hydroxylated derivatives, such as 7-hydroxycoumarin, in angular pyranocoumarins. Conclusion The UPLC-MS/MS method devised in this study has proved to be highly efficient and precise. Along with chemometric models, this method can shed light on the species-specific distribution profiles of coumarins in Peucedanum medicinal materials. This approach offers a multi-index collaborative control strategy as well as data-driven means for the varietal authentication, quality standardization, and regulatory supervision of traditional Chinese medicines.

Key words: Peucedanum praeruptorum Dunn, Peucedanum decursivum (Miq.) Maxim., Praeruptorin A, Praeruptorin B, Nodakenin, Coumarin, UPLC-MS/MS, Chemometrics

CLC Number:

R917

LI Yaolei, JIN Hongyu, CHENG Xianlong, WEI Feng. Quantitative Characterization and Chemometric Discrimination of 24 Coumarin Constituents in Peucedanum Medicinal Materials[J]. Chinese Journal of Pharmacovigilance, 2025, 22(7): 755-762.

Add to citation manager EndNote|Ris|BibTeX

URL: https://zgywjj.magtechjournal.com/EN/10.19803/j.1672-8629.20250213

https://zgywjj.magtechjournal.com/EN/Y2025/V22/I7/755

References

[1] LI Y, JIN H, WEI F, et al.Analysis of General Information and Quality Factors of Peucedanum praeruptorum Dunn[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2024, 21(11): 1315-1320.
[2] WANG Q, SUN Q, HUANG Q, et al.The Traditional Uses, Pharmacology, and Phytochemistry of Peucedanum praeruptorum Dunn[J]. Front Pharmacol, 2024, 15(10): 1352657.
[3] LIANG T, HONG J, SHANG Z.The Record of Famous Doctors(名医别录)[M]. Beijing: People’s Health Publishing House, 1986: 122.
[4] SONG Z, LI B, TIAN K, et al.Research Progress on Chemical Constituents and Pharmacological Activities of Peucedani Radix and Peucedani Decursivi Radix[J]. Chinese Herbal Medicine(中草药), 2022, 53(3): 948-964.
[5] Chinese Pharmacopoeia Commission.Pharmacopoeia of the People’s Republic of China,Volume I(中华人民共和国药典一部)[M]. Beijing: China Medical Science Press, 2025.
[6] CHEN Q, LIU X, PAN L, et al.Study on the Effects of Praeruptorin A Combined with Low Concentration of Taxol on Taxol-Resistant Ovarian Cancer Cell Line A2780/TAX[J]. Chinese Journal of Hospital Pharmacy(中国医院药学杂志), 2022, 42(17): 1755-1759, 1765.
[7] GUAN F, JIN W, ZHANG X, et al.The Effects of Praeruptorin A on the Contraction of Isolated Tracheal Smooth Muscle Previously Induced by High K and Acetylcholine[J]. Journal of China Medical University(中国医科大学学报), 1994, 23(6): 549-552.
[8] YU CL, YU YL, YANG SF, et al.Praeruptorin A Reduces Metastasis of Human Hepatocellular Carcinoma Cells by Targeting ERK/MMP1 Signaling Pathway[J]. Environmental Toxicology, 2021, 36(4): 540-549.
[9] ZHENG ZG, LU C, THU PM, et al.Correction: Praeruptorin B Improves Diet-Induced Hyperlipidemia and Alleviates Insulin Resistance via Regulating SREBP Signaling Pathway[J]. RSC Advances, 2019, 9(11): 6101.
[10] LIN CL, HUNG TW, YING TH, et al.Praeruptorin B Mitigates the Metastatic Ability of Human Renal Carcinoma Cells through Targeting CTSC and CTSV Expression[J]. International Journal of Molecular Sciences, 2022, 21(8): 2919.
[11] OZAKI H, NISHIDONO Y, FUJII A, et al.Identification of Anti-Inflammatory Compounds from Peucedanum praeruptorum Roots by Using Nitric Oxide-Producing Rat Hepatocytes Stimulated by Interleukin 1β[J]. Molecules, 2023, 28(13): 5076.
[12] LI Y, WU H, FAN J, et al.Machine Learning Sensing Coupled with Critical Components Elucidates Peucedanum praeruptorum Dunn Quality under Multi-Factors[J]. Microchemical Journal, 2025, 211: 113054.
[13] XIE J, TANG X, XIE C, et al.Comparative Analysis of Root Anatomical Structure, Chemical Components and Differentially Expressed Genes between Early Bolting and Unbolting in Peucedanum praeruptorum Dunn[J]. Genomics, 2023, 115(2): 110557.
[14] LI Y, LIN Z, WANG Y, et al.Unraveling the Mystery of Efficacy in Chinese Medicine Formula: New Approaches and Technologies for Research on Pharmacodynamic Substances[J]. Arabian Journal of Chemistry, 2022, 15(11): 104302.
[15] GAO L, ZHANG L, FENG T, et al.An AI-Driven Strategy for Active Compounds Discovery and Non-Destructive Quality Control in Traditional Chinese Medicine: a Case of Xuefu Zhuyu Oral Liquid[J]. Talanta, 2025, 287: 127627.
[16] GAO L, WANG X, MA JM, et al.Simultaneous Determination of Fifteen Components in Xuanfudaizhe Decoction by UPLC-MS/MS[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2023, 20(9): 987-991, 1006.
[17] LI YY, WANG Y, ZHANG NP et al. Effects of Different Harvest Seasons on Anthraquinone Contents of Polygoni Multiflori Radix Based on UPLC-MS/MS[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2022, 19(12): 1277-1284.
[18] CHEN J, NIE L, CHENG X, et al.Qualitative and Quantitative Analysis of Main Characteristic Components of Glycyrrhiza Inflate Bat. with Chemometrics[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2021, 18(9): 826-830.
[19] LI YY, WANG Q, YANG JB, et al.Influences of Growth Years and Harvest Seasons on Content of 14 Components in Polygoni Multiflori Radix[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2023, 20(8): 1769-1775.
[20] LI YY, WEN HR, WANG Y, et al.Influences of Continuous Steaming with Black Bean Decoction on Contents of 24 Components of Polygoni Multifori Radix[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2023, 20(6): 609-615.
[21] LI Y, LU H, JIA B, et al.Bioinformatics Analysis of Key Enzyme Genes Involved in Coumarin Biosynthetic Pathway of Peucedanum praeruptorum Dunn under Salt Stress[J]. Journal of Anhui University of Chinese Medicine(安徽中医药大学学报), 2022, 41(4): 87-91.
[22] JU S, LI Y, LIN Z, et al.Present Situation Analysis and Thinking on Quality Control Mode of Chinese Materia Medica[J]. Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学), 2022, 28(18): 269-274.
[23] HUANG C, TANG H, WEI X, et al.The Gradual Establishment of Complex Coumarin Biosynthetic Pathway in Apiaceae[J]. Nature Communications, 2024, 15(1): 6864.