The TLC characteristic analysis of cattle bile and sheep bile based on RGB multichannel data of images

doi:10.19803/j.1672-8629.20210403

Abstract

Abstract: Objective To investigate the Thin-layer Chromatography (TLC) characteristic of cattle bile and sheep bile. Methods TLC method was developed with isooctane-ether-glacial acetic acid-n-butanol-water (6:5:5:3:1) as developer, 10% sulfuric acid ethyl alcohol solution as chromogenic reagent. After heating at 105 ℃ until spots marked, TLC was checked and stored as a image. Then RGB multichannel data of TLC image were extracted out and analyzed by Hierarchical Cluster Analysis (HCA), Principal Components Analysis (PCA) and Orthogonal Projections to Latent Structures - Discrimination Analysis (OPLS-DA). Results The data of channel of B had the most characteristic for the difference of cattle bile and sheep bile. In TLC, spots of glycocholic acid, glycidodeoxycholic acid and spot 4 (unknown) were markers for distinguishing cattle bile and sheep bile. Conclusion The TLC analysis method could be a reference due to its simplicity, sensitivity and accuracy compared with traditional TLC method.

Key words: cattle bile, sheep bile, TLC, identification, chemometrics

CLC Number:

R917.1

SHI Yan, WEI Feng, MA Shuangcheng. The TLC characteristic analysis of cattle bile and sheep bile based on RGB multichannel data of images[J]. Chinese Journal of Pharmacovigilance, 2022, 19(10): 1060-1066.

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