Effects of duration of steaming on 26 chemical compositions of Polygoni Multiflori Radix based on UPLC-MS/MS detection technology

doi:10.19803/j.1672-8629.20220458

Abstract

Abstract: Objective To explore the effects of duration of steaming (0~48 h) and processing methods recorded in “Chinese Pharmacopoeia” on the contents of main chemical components of Polygoni Multiflori Radix（PM）. Methods Pharmacopoeia-defined steamed samples were prepared before an ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) analytical method was established for determination of the 26 components in PM. Results An accurate and reliable UPLC-MS/MS method was established for the determination of anthraquinone, stilbene glycosides, flavonoids and phenolic compounds in PM. With the extension of steaming time, the contents of 26 components changed significantly. The contents of free anthraquinone components (emodin, physcion) decreased before they increased, while those of combined anthraquinone components (emodin-8-O-β-D-glucoside, Physcion- -8-O-β-D-glucoside, emodin-1-O-glucoside, emodin-6-O-glucoside), rhein, hydroxyemodin, emodin-1-methyl, cis-THSG, polydatin, epicatechin and rutin increased first and then decreased. The contents of emodin dianthrone, emodin-8-O-(6’-methylmalonyl)-glucopyranoside, physcion-8-O-(6’- methylmalonyl)-glucopyranoside, trans-THSG, resveratrol, 2,3,5,4’-tetrahydroxystilbene-2-0-(2”-O-feruloyl)-β-D-glucopyranoside, catechin and torachrysone-8-O-β-D-glucopyranoside decreased, while those of phenolic components increased. Conclusion This method is simple, accurate and repeatable, which can be used for quantitative detection and analysis of 26 chemical components in PM. The contents of the 26 components in PM are closely related to the duration of steaming. Anthraquinones, stilbene glycosides, flavonoids and phenols undergo regular changes, which have an impact on the quality of PM.

Key words: Polygoni Multiflori Radix, Polygonum Multiflorum Radix preparata, UPLC-MS/MS, anthraquinones, stilbene glycosides, flavonoids, phenols

CLC Number:

R927.2

LI Yanyi, WANG Ying, ZHANG Nanping, YANG Jianbo, LIU Yue, WANG Qi, ZHANG Yujie, WEI Feng, MA Shuangcheng. Effects of duration of steaming on 26 chemical compositions of Polygoni Multiflori Radix based on UPLC-MS/MS detection technology[J]. Chinese Journal of Pharmacovigilance, 2022, 19(12): 1295-1302.

References

[1] TEKA TEKLEAB, WANG LM, GAO J, et al.Polygonum multiflorum: recent updates on newly isolated compounds, potential hepatotoxic compounds and their mechanisms[J]. Journal of Ethnopharmacology, 2021, 271: 113864.
[2] LIU SB.Effects of different processing technology on efficacy and activity of Polygonum multiflorum Thunb.[J]. Chinese Journal of Clinical Rational Drug Use(临床合理用药杂志), 2017, 10(19): 100-101.
[3] LI YY, ZHANG YJ, WANG Q, et al.Research progress on the mechanism of hepatotoxicity associated with Polygonum multiflorum Thunb.[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2022, 19(6): 605-609.
[4] Chinese Pharmacopoeia Commission.Pharmacopoeia of the People’s Republic of China, Volume I, 2020 edition (中华人民共和国药典一部,2020年版)[M]. Beijing: China Medical Science and Technology Press, 2020:183-184.
[5] LI YY, ZHANG YJ, WANG Q, et al.Discussion on the chemical composition changes of Polygonum multiflorum Thunb. based on the origin and processing technology[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2022, 19(7): 799-802.
[6] HE Y.Effects of long-term administration of rhubarb on colon and its microecology in rats[D]. Xi’an: Northwest University, 2019.
[7] WANG Q, ZHANG QH, WEN HR, et al.Study on potential hepatotoxicity of main monomers of Polygonum multiflorum Thunb. based on liver microstructure[J]. Chinese Journal of Traditional Chinese Medicine(中国中药杂志), 2020, 45(12): 2954-2959.
[8] WANG Q, WEN HR, MA SC.Screening of hepatotoxic components in Polygonum multiflorum by taking FXR nuclear receptors as targets[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2022, 19(6): 630-634.
[9] WANG Q, YAN M, MA SC, et al.Predicting toxic monomer components in Polygonum multiflorum based on quantitative structure-activity relationship[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2021, 18(4): 352-355, 360.
[10] DONG LH, GUO PP, YAN WY, et al.Comparative study on hypolipidemic effect of cis-2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside and trans-2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside[J]. Journal of Shenyang Pharmaceutical University(沈阳药科大学学报), 2014, 31(12): 989-993.
[11] SUN M, YU QW, XIANG T, et al.2,3,5,4’-Tetrahydroxystibane-2-O-β-D-glucoside induces liver injury by disrupting bile acid homeostasis and phospholipids efflux[J]. Chinese Journal of Traditional Chinese Medicine(中国中药杂志), 2021, 46(1): 139-145.
[12] MA ZJ.Preliminary study on objectivity, clinical markers and injury mechanism of hepatotoxicity of Polygonum multiflorum Thunb.[D]. Chengdu: Chengdu University of TCM, 2013.
[13] GAO D, LI XF, YIN P, et al.Preliminary study on hepatotoxic components in Polygoni Multiflori Radix based on processing and toxicity-decreasing[J]. Chinese Traditional and Herbal Drugs(中草药), 2017, 48(10): 2044-2050.
[14] LI XF.Study on processing and detoxification of Polygonum multiflorum Thunb. Based on integrated evaluation and control of quality, effect and Application[D]. Jinan: Shandong University of Traditional Chinese Medicine, 2015.
[15] LIU YL, GAO D, LI XF, et al.Preliminary study on hepatotoxic components in Polygoni Multiflori Radix based on different adjuvants processing for detoxification[J]. Chinese Traditional and Herbal Drugs(中草药), 2020, 51(2): 330-337.