Uncertainty Evaluation for the Determination of Tacrolimus Content in Human Whole Blood by UPLC-MS/MS Method

doi:10.19803/j.1672-8629.20240850

Abstract

Abstract: Objective To evaluate the uncertainty of human whole blood tacrolimus (TAC) by ultra-performance liquid chromatography-mass spectrometry coupled with immunosuppressant kits. Methods The source of uncertainty in the concentration of TAC in human whole blood was analyzed and synthesized by UPLC-MS/MS. Results The extended uncertainty of a low concentration (3.54 mg·L^-1) and a high concentration (14.89 mg·L^-1) of TAC in human whole blood was 0.177 8 mg·L^-1 and 0.7 mg·L^-1, respectively (P=95%, k=2). Conclusion In this study, we have explored the establishment of a systematic evaluation standard for applications of uncertainty evaluation methods to immunosuppre-ssant kits,which can effectively identify the key error sources in the measurement process and provide a reference for the improvement and optimization of the kits.

Key words: Tacrolimus, UPLC-MS/MS, Content Determination, Uncertainty Evaluation, Whole Blood

CLC Number:

R917

WANG Jiaqing, WANG Qi, LIU Tongtong, ZHANG Dongjie, NING Xiao. Uncertainty Evaluation for the Determination of Tacrolimus Content in Human Whole Blood by UPLC-MS/MS Method[J]. Chinese Journal of Pharmacovigilance, 2025, 22(4): 386-390.

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URL: https://zgywjj.magtechjournal.com/EN/10.19803/j.1672-8629.20240850

https://zgywjj.magtechjournal.com/EN/Y2025/V22/I4/386

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