Uncertainty evaluation for the determination of 6-MP in Beagle dog plasma by UPLC-MS/MS

doi:10.19803/j.1672-8629.20230593

Abstract

Abstract: Objective To evaluate the uncertainty for the determination of 6-mercaptopurine (6-MP) in beagle dog plasma by UPLC-MS/MS. Methods The whole process during the 6-MP determination was analyzed and the uncertainty from various sources were calculated. The combined and expanded uncertainty were also evaluated. Results The expanded uncertainty of 6-MP at low (15 ng·mL^-1 ) and high (375 ng·mL^-1) levels were 1.04 ng·mL^-1and 30.14 ng·mL^-1, respectively (P = 95%, k = 2). Conclusion The uncertainty for the determination of 6-MP in beagle dog plasma by UPLC-MS/MS at low concentration is mainly caused by calibration curve fitting, recovery and matrix effect, while at high concentration the uncertainty is mainly caused by recovery, calibration curve fitting and sample preparation.

Key words: UPLC-MS/MS, 6-MP, determination, uncertainty, Beagle dog, plasma

CLC Number:

R927.1

WANG Jiaqing, WANG Qi, SHI Weizhong, ZHANG Dongjie, NING Xiao. Uncertainty evaluation for the determination of 6-MP in Beagle dog plasma by UPLC-MS/MS[J]. Chinese Journal of Pharmacovigilance, 2023, 20(12): 1321-1325.

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