Determination of Linezolid Concentrations in Plasma with HPLC-MS/MS Method

doi:10.19803/j.1672-8629.20240848

Abstract

Abstract: Objective To establish an HPLC-MS/MS method for determining the concentration of linezolid in plasma. Methods Linezolid-D₃ was used as the internal standard. The sample release agent containing the internal standard was added. Protein was precipitated with methanol before the supernatant was injected for detection. Results Linezolid showed good linearity within the range of 0.42-28 μg·mL^-1. The average recovery was 106.7% for the low concentration, 104.9% for the medium concentration, and 105.9% for the high concentration. The intra-day and inter-day precision was 2.38 %-6.74 % and 1.37 %-2.78 %, respectively, and the precision ranged from 1.37 % to 2.78 %. Conclusion This method is simple and fast, which can be used for rapid clinical monitoring of blood drug concentrations.

Key words: Linezolid, Liquid Chromatography-Mass Spectrometry, Therapeutic Drug Monitoring, Plasma, Bovine Serum

CLC Number:

R917

WANG Jiaqing, WANG Qi, LIU Tongtong, ZHANG Dongjie, CHEN Qi, MEI Shenghui, NING Xiao. Determination of Linezolid Concentrations in Plasma with HPLC-MS/MS Method[J]. Chinese Journal of Pharmacovigilance, 2025, 22(4): 377-380.

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URL: https://zgywjj.magtechjournal.com/EN/10.19803/j.1672-8629.20240848

https://zgywjj.magtechjournal.com/EN/Y2025/V22/I4/377

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