Determination of Chlorogenic Acid in Fufang Shengdi Mixture by HPLC

Abstract

Abstract: Objective To establish a HPLC method for the determination of chlorogenic acid in Fufang Shengdi mixture. Methods A SHISEIDO MG C18 column(250 mm×4.6 mm, 5μm) was used with the mobile phase of 0.4% phosphoric acid and acetonitrile for isocratic elution (10:90) at the wavelength of 327 nm, with the flow rate of 1mL·min-1 and the column temperature was 30℃. Results The calibration curves for chlorogenic acid were linear in the concentration ranges of 0.0624～4.68 μg, and the correlation coefficient was 0.999999. The average recovery was 100.7%,(RSD=1.7%,n=6). Conclusion This method is simple and reproducible,and is suitable for the quality control of the Fufang Shengdi mixture.

Key words: high pressure liquid chromatography(HPLC), Fufang Shengdi mixture, chlorogenic acid

CLC Number:

R917

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XU Wei-ying, ZHENG Xin-ying, GU Xiao-ying, REN Shi-he. Determination of Chlorogenic Acid in Fufang Shengdi Mixture by HPLC[J]. Chinese Journal of Pharmacovigilance, 2014, 11(11): 661-663.

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References

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Determination of Chlorogenic Acid in Fufang Shengdi Mixture by HPLC

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[1]	LIU Yi, WU Jingfang, QIN Lin, CHEN Junmiao, ZHANG Juan, CHEN Hua, NING Xiao, CAO Jin. Related Substances in Efavirenz Tablets Based on HPLC-TRIPLE QUAD-MS/MS Technology [J]. Chinese Journal of Pharmacovigilance, 2025, 22(7): 749-754.
[2]	LI Yaolei, JIN Hongyu, CHENG Xianlong, WEI Feng. Quantitative Characterization and Chemometric Discrimination of 24 Coumarin Constituents in Peucedanum Medicinal Materials [J]. Chinese Journal of Pharmacovigilance, 2025, 22(7): 755-762.
[3]	WANG Jiaqing, WANG Qi, LIU Tongtong, ZHANG Dongjie, CHEN Qi, MEI Shenghui, NING Xiao. Determination of Linezolid Concentrations in Plasma with HPLC-MS/MS Method [J]. Chinese Journal of Pharmacovigilance, 2025, 22(4): 377-380.
[4]	JIN Shaoming, NING Xiao. Uncertainty Evaluation for Determination of Ambroxol in Rat Plasma by UPLC-MS/MS Method [J]. Chinese Journal of Pharmacovigilance, 2025, 22(4): 381-385.
[5]	WANG Jiaqing, WANG Qi, LIU Tongtong, ZHANG Dongjie, NING Xiao. Uncertainty Evaluation for the Determination of Tacrolimus Content in Human Whole Blood by UPLC-MS/MS Method [J]. Chinese Journal of Pharmacovigilance, 2025, 22(4): 386-390.
[6]	CHEN Huan, LIANG Jun, ZHAO Wei, XIA Qingrong. Clinical Features and Risk Factors of Liver Injury Induced by Atypical Antipsychotic Drugs [J]. Chinese Journal of Pharmacovigilance, 2025, 22(4): 410-414.
[7]	LIU Bo, YUAN Yanan, WANG Yue, CHENG Shuqian, LU Zhiguo, FAN Huihong, SUN Huimin. Key Technologies for Quality Control of Oligonucleotide Drugs [J]. Chinese Journal of Pharmacovigilance, 2025, 22(1): 1-9.
[8]	SUN Deyang, SUN Jiabei, LI Wenlong, JIA Juanjuan, ZHANG Wenzai, LIU Yan, HUANG Haiwei, YAO Jing. Assignment of Chemical Reference Substance of Aminopolyether -a Key Impurity in Fludeoxyglucose [¹⁸F] Injection [J]. Chinese Journal of Pharmacovigilance, 2025, 22(1): 43-46.
[9]	YAO Jing, LIAN Xiaofang, ZUO Limin, GUO Xin, LIU Huiyi, GU Yongsheng, JIA Qingying, HUANG Haiwei. Determination of Contents of 15 Elemental Impurities in Kits of Dimercaptosuccinate Acid and Stannous Chloride for Injection by ICP-MS [J]. Chinese Journal of Pharmacovigilance, 2025, 22(1): 53-57.
[10]	ZAN Ke, CAI Xisheng, WU Yun, WANG Zhao, WANG Dandan, LI Hailiang, JIN Hongyu. Six Active Ingredients in Medicinal and Non-Medicinal Parts of Farfarae Flos: a Comparative Study [J]. Chinese Journal of Pharmacovigilance, 2025, 22(1): 67-71.
[11]	DAI Mengru, LI Chun, ZHANG Yongxin, LU Yu, CHENG Boyu, FENG Weihong. Study on Catalpol Determination in Rehmanniae Radix [J]. Chinese Journal of Pharmacovigilance, 2025, 22(1): 76-83.
[12]	YANG Yanrong, KANG Shuai, LI Yanyi, MA Shuangcheng, WEI Feng, WANG Fang, WANG Qi. Research Progress in Quality Evaluation of Polygonum multiflorum Thunb. Based on Features of Traditional Identification [J]. Chinese Journal of Pharmacovigilance, 2024, 21(12): 1357-1362.
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[14]	WANG Qi, YANG Yanrong, LI Yanyi, XIE Jingxin, WEI Feng, MA Shuangcheng, WANG Fang, SHI Jia. Tissue Distribution of Chemical Components Based on Characters of Polygonum multiflorum Thunb. [J]. Chinese Journal of Pharmacovigilance, 2024, 21(12): 1371-1376.
[15]	LI Yanyi, WEN Hairuo, YANG Yanrong, MA Shuangcheng, WANG Qi. Identification of Hepatotoxic Components of Polygoni Multiflori Radix Based on Material Basis Combined with Heparg Cell Experiments [J]. Chinese Journal of Pharmacovigilance, 2024, 21(12): 1377-1381.