Abstract: Objective To qualitatively analyze the chemical compositions of Huatie compound and predict the potential mechanism of Huatie compound in the prevention and treatment of breast hyperplasia, so as to lay a foundation for further revealing the pharmacodynamic material basis of Huatie compound and its potential molecular mechanism in the prevention and treatment of breast hyperplasia. Methods UPLC-Orbitrap Exploris-MS technology was used to perform full scan detection in positive and negative ion modes. The chemical compositions of Huatie compound extract (80 % methanol as solvent) were analyzed by CNKI, SciFinder, PubChem, TCMSP and other databases. The network pharmacology method was used to screen the active ingredients through drug-likeness and oral bioavailability, and the SwissTargetPrediction database was used to predict the target of the compound components, and the OMIM database was used to obtain the target of breast hyperplasia. String online database was used to construct protein-protein interaction (PPI) network diagram. Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed through DAVID database, and Cytoscape 3.8.0 software was used to construct a network diagram of ‘Chinese medicine-active ingredient-target-signal pathway. Results 101 compounds were identified from Huatie compound, including 22 terpenoids, 22 flavonoids, 14 phenylpropanoids, 10 phenols, 9 alkaloids, 9 amino acids, 4 esters, 4 fatty acids, 4 fatty acids, 2 xanthones and 1 organic acid. Twenty-four active components were selected according to drug-like properties (DL≥0.18) and oral bioavailability (OB≥30%), and 2 003 breast hyperplasia-related target genes were retrieved through the OMIM database, and 250 breast hyperplasia targets of Huatie compound were obtained by intersection. PPI network analysis showed that 9 core network proteins included TNF, VEGFA, PI3KCA, PI3KR1, MAPK1, EGFR, AKT1, SRC and HSP90AA1. Conclusion The results of network analysis showed that the treatment of hyperplasia of mammary glands with Huatie compound was related to biological processes such as protein phosphorylation, signal transduction, and protein autophosphorylation. Abundant chemical components in Huatie compound may play a role in preventing and treating hyperplasia of mammary glands by regulating MAPK, Ras and other signaling pathways.

Key words: Huatie compound, Broussonetia Fructus, Clematidis Radix et Rhizoma, hyperplasia of mammary glands, chemical composition, UPLC-MS, network pharmacology