恶性肿瘤患者T淋巴细胞亚群及NK细胞活性与抗菌药物使用相关性

中国药物警戒 ›› 2016, Vol. 13 ›› Issue (11): 645-650.

恶性肿瘤患者T淋巴细胞亚群及NK细胞活性与抗菌药物使用相关性

张红旭，韩枫，黄金岳，凌心，刘广锋

徐州市肿瘤医院,江苏徐州 221005

收稿日期:2016-12-05 修回日期:2016-12-05 出版日期:2016-11-20 发布日期:2016-12-05
作者简介:张红旭,男,硕士,副主任药师,医院药学。E-mail:wastlx@163.com

Correlation between Activity of T Lymphocyte Subsets and NK Cells and Antibiotics Use in the Cancer Patients

ZHANG Hong-xu, HAN Feng, HUANG Jin-yue, LING Xin, LIU Guang-feng

Xuzhou Tumour Hospital, Jiangsu Xuzhou 221005, China

Received:2016-12-05 Revised:2016-12-05 Online:2016-11-20 Published:2016-12-05
Supported by:
江苏省徐州市社会发展科技计划项目（XZZDY1405）

摘要/Abstract

摘要： 目的研究恶性肿瘤患者T淋巴细胞亚群及NK细胞活性与抗菌药物使用的相关性。方法采用流式细胞仪检测恶性肿瘤患者及健康体检者外周血T淋巴细胞亚群、NK细胞活性;采用t 检验、χ²检验、单因素方差分析（F 检验）、SNK-q 检验及Pearson相关性分析等分析方法进行统计学分析。结果总体比较：病例组外周血中CD3⁺、CD4⁺、NK细胞比例显著减少,CD8⁺比例显著增加,CD4⁺/CD8⁺比值明显下降,与对照组比较,差异有统计学意义(均为P<0.05)。相关性分析：病例组外周血中CD3⁺、CD4⁺、CD8⁺、NK细胞比例、CD4⁺/CD8⁺比值与抗菌药物累积使用量呈低度相关,相关有统计学意义(r=-0.36、-0.40、0.38、-0.38、-0.36,均为P<0.05)。不同因素对健康人外周血T淋巴细胞亚群、NK细胞表达的影响：性别、年龄对健康成年人胃肠道中的细菌总数、乳酸菌数量的影响未达到统计学意义（P>0.05）。结论在一定时间段内,抗菌药物累积使用达到一定量后,恶性肿瘤患者T淋巴细胞亚群及NK细胞活性与抗菌药物使用存在低度相关性。

关键词: 恶性肿瘤, T淋巴细胞亚群, NK细胞, 活性, 抗菌药物, 相关性

Abstract: Objective To study the correlation between the activity of T lymphocyte subsets, NK cells and antibiotics use in cancer patients. Methods The activity of T lymphocyte subsets and NK cells in the peripheral blood of the cancer patients and healthy controls were detected by using flow cytometry. The correlation between the activity of probiotics and antibiotics use in cancer patients was statistically analyzed by t-test, chi-square test, one-way analysis of variance ( F-test), Student-Newman-Keuls q-test and Pearson correlation analysis. Results Overall comparison: the percentage of CD3⁺, CD4⁺, NK cells, and CD4⁺/CD8⁺ ratio in peripheral blood of the cancer patients were decreased significantly, while the percentage of CD8⁺ was significantly increased, compared with the normal control group. The difference was statistically significant (each P < 0.05). Correlation analysis: there was weakly correlation between the percentage of CD3⁺, CD4⁺, CD8⁺, NK cells, and CD4⁺/CD8⁺ ratio in peripheral blood and the cumulative amount of antibiotics use of the case group ( r= -0.36, -0.40, 0.38, -0.38, -0.36, each P < 0.05). The effects of different factors on expression of T lymphocyte subsets and NK cells in peripheral blood of the healthy controls: the effect of both gender and age on the total number of bacteria and the number of lactobacillus in gastrointestinal tract of the healthy controls dose not reach statistical significance ( P > 0.05). Conclusion There is a weakly correlation between activity of T tymphocyte subsets, NK cells and antibiotics use in cancer patients after antibiotics cumulative use reaches a certain amount in a certain period of time.

Key words: cancer, T lymphocyte subset, NK cell, activity, antibiotic, correlation

中图分类号:

R969.3

张红旭，韩枫，黄金岳，凌心，刘广锋. 恶性肿瘤患者T淋巴细胞亚群及NK细胞活性与抗菌药物使用相关性[J]. 中国药物警戒, 2016, 13(11): 645-650.

ZHANG Hong-xu, HAN Feng, HUANG Jin-yue, LING Xin, LIU Guang-feng. Correlation between Activity of T Lymphocyte Subsets and NK Cells and Antibiotics Use in the Cancer Patients[J]. Chinese Journal of Pharmacovigilance, 2016, 13(11): 645-650.

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