中国药物警戒 ›› 2025, Vol. 22 ›› Issue (1): 67-71.
DOI: 10.19803/j.1672-8629.20240569

• 基础与临床研究 • 上一篇    下一篇

款冬花药用与非药用部位中6种活性成分比较研究

昝珂1, 蔡喜生2,∆, 吴芸3, 王赵1, 王丹丹1, 李海亮1#, 金红宇1,*   

  1. 1中国食品药品检定研究院中药民族药检定所,北京 102629;
    2通化市食品药品检验所中药室,吉林 通化 134001;
    3泰州市药品检验院中药室,江苏 泰州 225300
  • 收稿日期:2024-08-12 出版日期:2025-01-15 发布日期:2025-01-22
  • 通讯作者: *金红宇,男,主任药师,中药质量安全评价。E-mail: jhyu@nifdc.org.cn#为共同通信作者。
  • 作者简介:昝珂,男,博士,副研究员,中药质量评价。△为并列第一作者。
  • 基金资助:
    国家重点研发计划（2022YFC3501505）; 中药材及饮片质量控制重点实验室开放基金（2023GSMPA-KL09）; 中国食品药品检定研究院关键技术研究基金（GJJS-2022-7-1）

Six Active Ingredients in Medicinal and Non-Medicinal Parts of Farfarae Flos: a Comparative Study

ZAN Ke1, CAI Xisheng2,∆, WU Yun3, WANG Zhao1, WANG Dandan1, LI Hailiang1#, JIN Hongyu1,*   

  1. 1National Institutes for Food and Drug Control, Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, Beijing 102629, China;
    2Tonghua Institutes for Food and Drug Control, Department of Traditional Chinese Medicine, Tonghua Jilin 134001, China;
    3Taizhou Institute for Drug Control, Department of Traditional Chinese Medicine, Taizhou Jiangsu 225300, China
  • Received:2024-08-12 Online:2025-01-15 Published:2025-01-22

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摘要： 目的 比较款冬花药用和非药用部位中6种活性成分含量差异。方法 建立高效液相色谱法同时测定款冬花不同部位中绿原酸、芦丁、3,4-O-二咖啡酰奎宁酸、3,5-O-二咖啡酰奎宁酸、4,5-O-二咖啡酰奎宁酸、款冬酮等6种成分的含量。采用ShimNex CS C18（4.6 mm×250 mm, 5 μm）色谱柱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,检测波长为220 nm,流速为1 mL·min-1,柱温为30 ℃。结果 芦丁和款冬酮在款冬花的花梗和根中均未检出,其余4种咖啡酰奎宁酸类成分也均低于花蕾。结论 验证了款冬花药材以花蕾入药的科学性和合理性,非药用部位的混入会影响该药材质量,建议在质量标准中增加杂质检查项目,确保药材的质量。

关键词: 款冬花, 药用部位, 非药用部位, 款冬酮, 活性成分, 3,5-O-二咖啡酰奎宁酸, 4,5-O-二咖啡酰奎宁酸, 高效液相色谱, 含量测定

Abstract: Objective To study the differences in contents of six active ingredients in medicinal and non-medicinal parts of Farfarae Flos. Methods A high-performance liquid chromatography method was established for simultaneous determination of six components, including chlorogenic acid, rutin, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, and tussilagone, in different parts of Farfarae Flos. The experiment was conducted using a ShimNex CS C18 (4.6 mm×250 mm, 5 μm) chromatographic column. The mobile phase was composed of acetonitrile-0.1% phosphoric acid aqueous solution under gradient elution. The detection wavelength was 220 nm at a flow rate of 1 mL·min-1 and the column temperature was 30℃. Results Rutin and tussilagone were not detected in the pedicels or roots of Farfarae Flos. The contents of other four caffeoylquinic acids were significantly lower than those in the buds. Conclusion The experiment has justified the use of buds of Farfarae Flos as the medicinal material. The mixture with non-medicinal parts will affect the quality of medicinal materials and should be avoided in the process of production. It is recommended that inspections of impurities be included in quality standards to ensure the quality of medicinal materials.

Key words: Farfarae Flos, Medicinal Part, Non-Medicinal Part, Tussilagone, Active Ingredient, 3,5-O-dicaffeoylquinic Acid, 4,5-O-dicaffeoylquinic Acid, HPLC, Content Determination

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