Abstract: Objective To investigate the pathologic injury degree of hepatotoxicity in mice induced by single administration of Yi Shen Wu Fa oral liquid and provide pathohistological basis for its hepatotoxical injury. Methods The pathologic changes of mice in the study on "dose-time-toxicity"relationship of hepatotoxicity caused by Yi Shen Wu Fa oral liquid was observed with light microscope and image analysis after HE stain. Results The study on the "time-toxicity" relationship indicated that the hepatotoxicity occurred at 2h after administration and expressed obviously in the range from 6h to 24h after administration. The study on the "dose-toxicity"relationship showed that pathohistological injury of different degree can be induced by drug of (10.56~25.00) g·kg-1 and acidophilia, hyalinization, vacuolus were the main pathologic changes. Conclusion Once oral administration of Yi Shen Wu Fa oral liquid with certain dosage may induce acute hepatotoxical injury in mice and show an obvious "dose-time-toxicity"relationship. It can cause pathologic changes such as acidophilia, hyalinization, vacuolus to hepatic cells.

Key words: Yi Shen Wu Fa oral liquid, hepatotoxicity, pathology, "dose-time-toxicity" relationship