Abstract: Objective To establish a method for determing 2, 3, 5, 4-tetrahydroxystibene-2-O-β-D-glucosid 、emodin、emodin monomethyl ether in yishen wufa oral liquids. To explore the relationship of toxicity of Yi Shen Wu Fa oral liquids and Radix Polygoni multiflori from the perspective of index constituents, and to provide method for the quality research of yishen wufa oral liquids. Methods HPLC, stationary phase was C18 column(4.6mm×250mm,5μm), mobile phase of 2,3,5,4′-tetrahydroxystibene-2-O-β-D-glucosid was acetonitrile-water solution(25∶75), detection wavelength 320nm, flow rate1.0mL·min-1 and column temperature25℃; mobile phase of emodin and emodin monomethly ether was methanol-0.1% phosphric acid(80:20), detection wavelength 254nm, flow rate1.0mL·min-1 and column temperature25℃. Results There was good linearity in the range of 0.1μg~10μg(r=0.9989) for 2,3,5,4′-tetrahydroxystibene-2-O-β-D-glucosid、in the range of 0.0168~1.68μg(r=0.9984) for emodin and in the range of 0.0208~1.04μg(r=0.9966)emodin monomethly ether; the average recovery of 2,3,5,4′-tetrahydroxystibene-2-O-β-D-glucosid、emodin、emodin monomethyl ether were 99.33%、99.998% and 99.576%, and RSD were 0.68%, 1.11 and 1.29%(n=5). Conclusion This method is simple, with high sensitivity and less interference, high reproducibility and recovery, can be used for quality control of Yi Shen Wu Fa oral liquids. It is initially confirmed that the toxicity of Yi Shen Wu Fa oral liquids may be related with the content of Stilbene glucoside、Emodin and Emodin monomethyl ether in Radix Polygoni multiflori. So, it should control the dose range of Yi Shen Wu Fa oral liquids and avoid adverse events.

Key words: HPLC, Yi Shen Wu Fa oral liquids, Stilbene glucoside, Emodin, Emodin monomethyl ether