Abstract: Objective Study the "dosage-time-toxicity" relationship of hepatotoxicity induced by Yi Shen Wu Fa oral liquid in mice. Methods Study on the "time-toxicity"relationship: Ig Yi Shen Wu Fa oral liquid of certain dosage to mice and the changes of the level of ALT, AST, TBI in serum and organ index of liver, spleen and thoracic gland were tested at different time points after administration. Study on the "dosage-toxicity"relationship: Ig Yi Shen Wu Fa oral liquid of different dosages to mice and the corresponding treatment was performed at 2h after administration in accordance with the method of study on"time-toxicity"relationship. Results The study on the "time-toxicity" relationship indicated that the level of ALT, AST, TBI in serum reached to the peak at 2h after once administration of Yi Shen Wu Fa oral liquid of higher dosage and the liver index rised to its topmost at 12h. They all recovered to normal value at 72h after ig. The study on the "dosage-toxicity"relationship indicated that conspicuous hepartotoxical injury can be induced by Yi Shen Wu Fa oral liquid of (4.46~25)mL·kg-1 and the level of ALT, AST, TBI increased significantly with the increase of dosage. Conclusion Once oral administration of Yi ShenWu Fa oral liquid with large dosage may induce acute hepatotoxical injury in mice and show an obvious "dosage-time-toxicity" relationship.

Key words: Yi Shen Wu Fa oral liquid, mice, hepatotoxicity, "dosage-time-toxicity" relationship