Hepatotoxicity of alcohol extract Polygoni Multiflori Radix and related monomer compositions in liver cells

doi:10.19803/j.1672-8629.2022.07.07

Abstract

Abstract: Objective To investigate the hepatotoxicity of 70% alcohol extract of Polygonum multiflorum, different eluted components and some monomer compounds isolated from the components in vitro. Methods The cell viability of HepG₂ and L02 cells were detected by MTT colorimetry. Hepatocyte apoptosis and cell cycle were analyzed by flow cytometry. Results Significant toxicity in HepG₂ cells was observed in 95% ethanol-eluted component (HSW-D) of 70% alcohol extract of Polygonum multiflorum and the monomer compound aloe emodin obtained from the component with 48 h treatment. Furthermore, significant toxicity of HSW-D was also presented in L02 cells after 48 h treatment. In addition, cell proliferation of HepG₂ and L02 cells were inhibited through S-phase arrest. In comparison, little toxicity in HepG₂ and L02 cells were observed in the 70% alcohol extract, other eluted components, two stilbene glycosides, two phenols and 10 monomer compounds such as chrysophanol, emodin methyl ether, rhein acid and emodin monanthrone of Polygonum polygonum. Conclusion The toxic substances were presented in the liposoluble constituent of alcohol extract of Polygonum multiflorum, by which aloe emodin is one of the essential toxic elements .

Key words: Polygoni Multiflori Radix, hepatotoxicity, Aloe emodin, cell cycle, liver cells

CLC Number:

R994.11

CHEN Zihan, YANG Jianbo, CHEN Zhiwei, MA Shuangcheng, WEI Feng, SUN Hua. Hepatotoxicity of alcohol extract Polygoni Multiflori Radix and related monomer compositions in liver cells[J]. Chinese Journal of Pharmacovigilance, 2022, 19(7): 728-732.

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