Abstract: Objective To make the preliminary study on analgetic effect mechanism of water extraction components of Evodia Fructus, and to discuss the relevance between the accompanying hepatotoxicity and oxidative damage mechanism. Methods Mice were randomly divided into 5 groups according to weight, namely normal group, diclofanac sodium sustained release tablets group, high, middle and low dose group of water extraction components of Evodia Fructus. Mice were continuously administrated for7 days, and the level of PGE2, MDA、SOD、NO、NOS、GSH、 GSH-Px in serum were detected. Results The water extraction components of Evodia Fructus could cause the level of PGE2 in serum decreased, the activity of MDA in serum increased, and the activity of SOD decreased. The level of NO and NOS were increased, and the activity of GSH and GSH-Px were decreased. All the changes were aggravated in accordance with the dosages, and there was sijnificant difference compared with distilled water control group and there was certain dose -dependence relationship. Conclusion The analgetic effect mechanism of water extraction components of Evodia Fructus was mainly related with the reduction of the content of PGE2 in serum, and the approach of accompanying hepatotoxicity damage was related with the peroxidative damage mechanism.

Key words: evodia fructus, water extraction components, analgetic effect mechanism, accompanying hepatotoxicity, mechanism