Abstract: Objective To explore the effect of ginsenoside Rd on chromosome aberration in Chinese hamster lung cells(CHL). Methods We used the method of cell counting to determine the IC50 of ginsenoside Rd on CHL cells, then to establish the range of doses according to the IC50 and to do the cell chromosome aberration experiment. When the CHL cells were exposured to ginsenoside Rd at 6h and 24h and plusedS9 mixture at 6h respectively, we observed the changes of chromosome number and structure, then to judge the chromosome aberration Results . Results Negative response was found at 6h and 24h after the treatment with ginsenoside Rd and at 6h after the addition of S9 mixture. Conclusion Under the condition of this experiment, ginsenoside Rd does not induce chromosome aberration in CHL cells.

Key words: ginsenoside Rd, Chinese hamster lung cells(CHL), chromosome aberration