Function and Mechanism of Polygalasaponin F on Amyloid β Protein-induced Damage in Mice

Abstract

Abstract: Objective To study the protective function and mechanism of polygalasaponin F(PGSF) against the injury induced by amyloid β protein (Aβ). Methods PC12 cells were exposed to Aβ, and the cell viability was analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT), the accumulation of intracellular reactive oxygen species(ROS) were measured by DCFH-DA probe, the effects of PGSF on cognition were evaluated through the Morris water maze, and the protein level was detected by Western blot methods. Results It was showed that PGSF could increase the cell viability-impaired by Aβ, and decrease the accumulation of ROS induced by Aβ. Results further showed that Aβ could prolong the escape latency and decrease the crossing frequency, and PGSF (20 mg·kg^-1) could improve these impaired movements. PGSF also could increase the protein level of brain derived neurophic factor(BDNF), and enhance the phosphorylation of TRK and GSK3β 216Y. Conclusion PGSF could protect cells from Aβ damage, and it was proved to prevent A-destroyed learning and memory, and this function might be associated with TRK-GSK3β-BDNF signal pathway. So, PGSF might be apotential compound for Alzheimer's disease.

Key words: polygalasaponin F, amyloid β protein, Alzheimer's disease, brain derived neurophic factor (BDNF)

CLC Number:

R965

GAO Wei-zhen,MU Xin,ZHANG Xiao-hua . Function and Mechanism of Polygalasaponin F on Amyloid β Protein-induced Damage in Mice[J]. Chinese Journal of Pharmacovigilance, 2017, 14(10): 577-581.

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