Interventions of Bushen Huoxue Decoction in Lumbar Intervertebral Disc Degeneration of Kidney Deficiency and Blood Stasis Type in Rats

doi:10.19803/j.1672-8629.20240939

Abstract

Abstract: Objective To explore the effect of interventions of Bushen Huoxue decoction in lumbar intervertebral disc degeneration with kidney deficiency and blood stasis in rats. Methods Thirty-six SD rats were randomly divided into the sham operation group, model group, meloxicam group (0.135 mg·kg^-1), and low, medium and high dose groups of Bushen Huoxue decoction (2.97、5.94、11.88 g·kg^-1), with 6 rats in each group. A lumbar disc degeneration model was established via lumbar dynamic and static imbalance while the model of kidney deficiency and blood stasis was established via intramuscular injection of hydrocortisone and subcutaneous injection of epinephrine. The pathomorphological changes of the lumbar intervertebral disc were observed by X-ray and safranine O-fast green (SO/FG) staining. The expressions of collagen typeⅡ (COL2A1), aggrecan (ACAN) and other proteins in the cartilage endplate of intervertebral discs of rats were observed by immunohistochemical(IHC) staining. The contents of serum glutamic oxalic aminotransferase (AST), alanine aminotransferase (ALT), urea nitrogen(BUN) and creatinine (CREA) were detected, the organ coefficients of the liver and kidney were calculated, and the pathological changes of liver and kidney tissues were observed by hematoxylin-eosin (HE) staining. Results Bushen Huoxue decoction could delay the degeneration of the intervertebral disc in rats, increase the expressions of COL2A1, ACAN, BCL-2 and RGCC in the cartilage endplate, and decrease the expression of BAX. The contents of serum ALT, AST, BUN and CREA as well as the organ coefficients and HE staining of the liver and kidney remained normal. Conclusion Bushen Huoxue decoction can not only help improve lumbar degeneration with kidney deficiency and blood stasis in rats, but delay the degeneration of the cartilage endplate of the intervertebral disc. This decoction can promote the expressions of collagen type II and aggrecan in the cartilage endplate, inhibit apoptosis and regulate cell cycle. It is safe for long-term interventions in lumbar intervertebral disc degeneration of kidney deficiency and blood stasis type.

Key words: Bushen Huoxue Decoction, Kidney Deficiency and Blood Stasis, Lumbar Intervertebral Disc Degeneration, Cartilage Endplate, Rats, Regulator of Cell Cycle RGCC, Safety

CLC Number:

R965

KANG Jiaqi, GUO Xiaofei, SU Hongmei, BAI Jianqi, ZHU Hongwei, BAI Xin, CHEN Xin, WANG Hao, ZHAO Xiaoqi, GUO Dongqi, WANG Yuan, ZHANG Ping. Interventions of Bushen Huoxue Decoction in Lumbar Intervertebral Disc Degeneration of Kidney Deficiency and Blood Stasis Type in Rats[J]. Chinese Journal of Pharmacovigilance, 2025, 22(3): 249-257.

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