中国药物警戒 ›› 2016, Vol. 13 ›› Issue (4): 206-211.

• 基础与临床研究 • 上一篇    下一篇

恶性肿瘤患者T淋巴细胞亚群及NK细胞活性与益生菌活性相关性研究

张红旭1,2,,刘广锋3,,韩枫2,,黄金岳2,,凌心2,,王艳1   

  1. 1徐州医学院附属医院药学部,江苏 徐州 221002 ;
    2徐州市肿瘤医院药剂科,江苏 徐州 221005 ;
    3徐州市肿瘤医院检验科,江苏 徐州 221005
  • 收稿日期:2016-02-25 修回日期:2016-06-30 出版日期:2016-04-20 发布日期:2016-06-30
  • 通讯作者: 王艳,女,主任药师,副教授·硕导,临床药学研究。
  • 作者简介:张红旭,男,硕士,副主任药师,医院药学。
  • 基金资助:
    江苏省徐州市社会发展科技计划项目（XZZDY1405）。

Study on Correlationship between Activity of T Lymphocyte Subsets and NK Cells and Activity of Probiotics in Cancer Patients

ZHANG Hong-xu1,2, LIU Guang-feng3, HAN Feng2, HUANG Jin-yue2, LING Xin2, WANG Yan1   

  1. 1Department of Pharmacy , Affiliated Hospital of Xuzhou Medical College, Jiangsu Xuzhou 221002, China;
    2Department of Pharmacy, Xuzhou Tumour Hospital, Jiangsu Xuzhou 221005, China;
    3Department of Clinical Laboratory , Xuzhou Tumour Hospital, Jiangsu Xuzhou 221005, China
  • Received:2016-02-25 Revised:2016-06-30 Online:2016-04-20 Published:2016-06-30
  • Contact: E-mail:xzwydd@163.com

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摘要： 目的 研究恶性肿瘤患者T淋巴细胞亚群及NK细胞活性与益生菌活性的相关性。方法 流式细胞仪检测恶性肿瘤患者及健康体检者外周血T淋巴细胞亚群、NK细胞活性; 平板计数法检测恶性肿瘤患者及健康体检者粪便中细菌总数和乳酸菌数量;两样本均数的t 检验、单因素方差分析（F 检验）、q 检验及相关性分析等分析方法进行统计学分析,并对相关系数进行检验。结果 细胞检测:总体比较,病例组外周血中CD3+、CD4+、NK细胞比例显著减少, CD8+比例显著增加, CD4+/CD8+比值明显下降,与对照组比较,差异有统计学意义（均为P<0.05）;分层比较,低天数组与对照组外周血中CD3+、CD4+、CD8+、NK细胞比例、CD4+/CD8+比值差异没有统计学意义（均为P>0.05）;中等天数组、高天数组与对照组外周血中CD3+、CD4+、CD8+、NK细胞比例、CD4+/CD8+比值差异有统计学意义（均为P<0.05、P<0.01）。细菌检测:总体比较,病例组细菌总数增加,而乳酸菌数量减少,与对照组比较,差异有统计学意义（P<0.05、P<0.05）;分层比较,低天数组与对照组在细菌总数和乳酸菌数量差异上没有统计学意义（P>0.05、P>0.05）;中等天数组、高天数组与对照组在细菌总数和乳酸菌数量差异上有统计学意义（P<0.05、P< 0.01）。相关性分析: 恶性肿瘤患者T淋巴细胞亚群、NK细胞活性与益生菌活性存在低度相关性（r=0.385、0.406、0.371、0.383、0.401,均为P<0.05）。结论 恶性肿瘤患者T淋巴细胞亚群、NK细胞活性与益生菌活性存在低度相关性。

关键词: 恶性肿瘤, 益生菌, 乳酸菌, T淋巴细胞亚群, NK细胞, 活性, 抗菌药物, 相关性

Abstract: Objective To study the correlationship between the activity of T lymphocyte subsets and NK cells and the activity of probiotics in cancer patients. Methods The activity of T lymphocyte subsets and NK cells in the peripheral blood of cancer patients and the healthy controls were detected by using flow cytometry. The total number of bacteria and the number of lactobacillus in the feces of cancer patients and the healthy controls were detected by using plate count method. The correlationship between the activity of probiotics, T lymphocyte subsets, and NK cells and antibiotics use in cancer patients was statistically analyzed by t-test of two sample mean,one-way analysis of variance(F-test), q-test and correlation analysis, and the correlation coefficients were also examined. Results In overall comparison of cell detecting, the percentage of CD3+, CD4+, NK cells, and CD4+/CD8+ ratio in peripheral blood of cancer patients decreased significantly, while the percentage of CD8+ significantly increased, compared with the normal control group, the difference was statistically significant(each P< 0.05). In stratified comparison of cell detecting, there was no statistical difference in the percentage of CD3+, CD4+, NK cells, and CD4+/CD8+ ratio in peripheral blood between the fewer days group and the control group(each P> 0.05). Compared with the normal control group, both the moderate days group and the more days group had statistical difference in the percentage of CD3+, CD4+, CD8+, NK cells , and CD4+/CD8+ ratio in peripheral blood(each P< 0.05; 0.01). In overall comparison of bacteria detecting, The total number of bacteria of the case group increased significantly,while the number of lactobacillus significantly decreased, compared with the normal control group, the difference was statistically significant(each P< 0.050). In stratified comparison of bacteria detecting, there was no statistical difference in the total number of bacteria and the number of lactobacillus between the fewer days group and the normal control group(each P> 0.05). Compared with the normal control group,both the more days group and the moderate days group had statistical difference in the total number of bacteria and lactobacillus(P< 0.05, P<0.01). with the correlation analysis, there was a weakly positive correlation between the activity of CD3+, CD4+, CD8+, NK cells, and CD4+/CD8+ ratio in peripheral blood and the activity of lactobacillus of the cancer patients(r=0.385, 0.406, 0.371, 0.383, 0.401, each P< 0.05). Conclusion There is a weakly positive correlation between the activity of T lymphocyte subsets, NK cells and the activity of probiotics in cancer patients.

Key words: cancer, probiotics, lactobacillus, T lymphocyte subsets, NK cells, activity, antibiotics, correlationship

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