中国药物警戒 ›› 2011, Vol. 8 ›› Issue (8): 462-464.

• 柴胡肝毒性物质基础研究 • 上一篇    下一篇

柴胡挥发油毛细管GC指纹图谱研究

孙玲1, 2, 杨倩3, 王金虎1, 2, 黄伟4, 孙蓉4, *   

  1. 1 山东省医药工业研究所,山东 济南250101;
    2 山东省化药重点实验室,山东 济南250101;
    3 山东中医药大学,山东 济南 250355;
    4 山东省中医药研究院,山东 济南 250014
  • 收稿日期:2011-03-21 出版日期:2011-08-10 发布日期:2015-07-30
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:孙玲,女,副主任药师,新药质量研究。
  • 基金资助:
    山东省科技攻关关键技术研究课题（2007GG2NS02073）; 山东省引进国外智力项目（L20083700336）; 国家自然科学基金课题（30672649）、（81073148）; 山东省科技平台建设项目（2008GG2NS02021）; 国家重点基础研究发展计划 （973）中医基础理论专项资助项目 （2009CB522802）

Study on Fingerprint of Volatile Oil from Bupleurum Chinense by Capillary GC

SUN Ling1, 2, YANG Qian3, WANG Jing-hu1, 2, HUANG Wei4, SUN Rong4, *   

  1. 1 Sandong Institute of Pharmaceutical Industry, Shandong Jinan 250101, China;
    2 Shandong Key Laboratory of Pharmaceutical, Shandong Jinan 2501011, China;
    3 Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
    4 Shandong Academy of Traditional Chinese Medicine, Shandong Jinan 250014, China
  • Received:2011-03-21 Online:2011-08-10 Published:2015-07-30

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摘要： 目的 运用毛细管气相色谱法建立柴胡挥发油的指纹图谱。方法 采用毛细管气相色谱法,对不同批次道地产区的柴胡挥发油进行指纹图谱分析,色谱条件：HP-5毛细管柱（30m×0.25mm×0.25μm）;程序升温：柱温50℃（保持10min）,以20℃·min-1升至240℃（保持40min）;气化室温度：250℃;载气：高纯氮气;体积流量：2.0mL·min-1;进样量：0.5μL;分流比 40 1。氢火焰离子化检测器（FID）,检测温度：250℃。结果 从6批道地药材产区的柴胡挥发油GC指纹图谱中确定16个共有峰,并进行不同产区柴胡相似度的比较,相似度大于0.9。结论 本方法简便、快捷、可靠,可用于柴胡挥发油的质量控制。

关键词: 柴胡, 挥发油, 毛细管气相色谱, 指纹图谱

Abstract: Objective To establish the fingerprint of volatile oil from Bupleurum Chinense by capillary GC. Methods The fingerprint analysis of volatile oil from Bupleurum Chinense was made by capillary GC. The analysis was performed on an HP-5(30m×0.25mm×0.25μm). The column was maintained at 50℃ for 10 min then elevated to 240℃ at 20℃·min-1 and maintained for 40 min. Gasifieation temperature：250℃, carry gas:N2(99.99%), flow rate:2.0mL·min-1, inlet volumn: 0.5μL; spliting ratio：(40：1), FID, injector temperature：250℃. Results The GC fingerprints were established and the 16 common peaks were found and identified with GC in volatile oil of 6 batches of Bupleurum Chinense. The comparison on similarity degree of the crude drugs from various habitats has been carried on and the correlative coefficient was over 0.9. Conclusion The capillary GC method is so simple and reliable that it is capable of effectively controlling the quality of Bupleurum Chinense.

Key words: Bupleurum Chinense, volatile oil, capillary GC, fingerprint

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