中国药物警戒 ›› 2011, Vol. 8 ›› Issue (8): 465-467.

• 柴胡肝毒性物质基础研究 • 上一篇    下一篇

柴胡中柴胡总皂苷及柴胡皂苷a的含量测定

朱兰兰1, 2, 孙玲3, 翟丽屏4, 黄伟1, 李素君5, 陆永辉1, 孙蓉1, *   

  1. 1 山东省中医药研究院,山东 济南 250014;
    2 天津中医药大学,天津 300193;
    3 山东省医药工业研究所,山东 济南 250101;
    4 山东省地方病研究所,山东 济南 250014;
    5 山东中医药大学,山东 济南 250355
  • 收稿日期:2011-03-10 出版日期:2011-08-10 发布日期:2015-07-30
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理研究。 E-mai：sunrong107@163.com
  • 作者简介:朱兰兰,女,在读硕士研究生,药物分析与中药毒代研究。
  • 基金资助:
    国家自然科学基金课题（30672649）、（81073148）; 山东省引进国外智力项目（L20083700336）; 山东省科技攻关关键技术研究课题（2007GG2NS02073）; 山东省科技平台建设项目（2008GG2NS02021）

Determination of the Content of Saikosaponins and Saikosaponin a in Bupleurum Chinense DC.

ZHU Lan-lan1, 2, SUN Ling3, ZHAI Li-ping4, HUANG Wei1, LI Su-jun5, LU Yong-hui1, SUN Rong1, *   

  1. 1 Shandong Academy of Chinese Medicine, Shandong Jinan 25014, China;
    2 Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    3 Shandong Institute of Pharmaceutical Industry, Shandong Jinan 250101, China;
    4 Shandong Institute for Prevention and Treatment of Endemic Disease, Shandong Jinan 250014, China;
    5 Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2011-03-10 Online:2011-08-10 Published:2015-07-30

PDF (PC)

716

摘要： 目的 建立柴胡中柴胡总皂苷及柴胡皂苷a的含量测定方法,并对样品进行含量测定。方法 采用紫外分光光度法,以柴胡皂苷a为对照品,在波长545nm处对样品中的总皂苷进行含量测定;采用高效液相色谱法,以C18色谱柱（4.6mm×250mm,5μm）、甲醇-水为流动相,流速为1mL·min-1,检测波长为210nm,测定样品中柴胡皂苷a的含量。结果 柴胡总皂苷在30~70μg/mL、柴胡皂苷a在50.4~252μg/mL范围内呈良好的线性关系,r分别为09956、0.9991;平均回收率：柴胡总皂苷为99.31%、柴胡皂苷a为99.22%;RSD：柴胡总皂苷为1.25%,柴胡皂苷a为1.07%。结论 本研究所建立的紫外分光光度法测定柴胡总皂苷及高效液相法测定皂苷a含量的方法,简便、易行、快速,结果准确可靠,适用于柴胡中柴胡总皂苷及柴胡皂苷a的含量测定,并为在安全剂量范围内正确使用柴胡提供依据。

关键词: 柴胡总皂苷, 柴胡皂苷a, 含量测定

Abstract: Objective To establish the method for determination of the content of saikosaponins and saikosaponin a in Bupleurum Chinense DC, and carries on determination of the content of the sample of saikosaponins and saikosaponin a. Methods Saikosaponin a was used as the chemical reference substance determine the content of saikosaponins by UV-Visible Spectrophotometry. The absorbency was measured at 545nm. The content of saikosaponin a in Bupleurum Chinense DC was determined by HPLC. The samples were separated by C18 column(4.6mm×250mm, 5μm). The mobile phase is methyl-alcohol-water(70:30). The flow is 1mL·min-1. The detector wavelength is 210 nm. Results There was good linearity in the range of 30~70μg/mL(r=0.9956) for saikosaponins、in the range of 50.4~252μg/mL (r=0.9991) for saikosaponin a; The average recovery of saikosaponins and saikosaponin a were 99.31% and 99.22%, and RSD were 1.25% and 1.07%(n=6). Conclusion The methods for determination of the content of saikosaponins by UV-Visible Spectrophotometry and saikosaponin a by HPLC were proved to be simple, easy and fast to perform, and the results obtained were reliable and suitable. So, it can be used for the quantitative determination of saikosaponins and saikosaponin a in Bupleurum Chinense DC.To provide the basis for correctly using Bupleurum Chinense DC in the safe dose scope.

Key words: Saikosaponins, Saikosaponin a, determination

中图分类号: