中国药物警戒 ›› 2024, Vol. 21 ›› Issue (11): 1256-1263.
DOI: 10.19803/j.1672-8629.20240470

• 基础与临床研究 • 上一篇    下一篇

基于UPLC-MS/MS法比较不同产地蒲公英叶中15种成分含量

雷京奇1, 张泽昭1,2,3, 任东1, 王鑫国1,2,3, 牛丽颖1,2,3,*   

  1. 1河北中医药大学,河北 石家庄 050091;
    2河北省中药配方颗粒技术创新中心,河北 石家庄 050091;
    3中药材品质评价与标准化河北省工程研究中心,河北 石家庄 050091
  • 收稿日期:2024-07-05 出版日期:2024-11-15 发布日期:2024-11-20
  • 通讯作者: *牛丽颖,女,教授·博导,中药分析与质量控制。E-mail: niuliyingyy@163.com
  • 作者简介:雷京奇,女,在读硕士,中药分析与药效物质基础。
  • 基金资助:
    河北省自然科学基金资助项目（H2022423335）; 国家中医药管理局科技项目（GZY-KJS-2023-030）; 河北省中医药管理局科研计划项目（2025075）

Determination of 15 Chemical Constituents in Dandelion Leaves from Different Areas of Production Based on UPLC-MS/MS

LEI Jingqi1, ZHANG Zezhao1,2,3, REN Dong1, WANG Xinguo1,2,3, NIU Liying1,2,3,*   

  1. 1Hebei University of Chinese Medicine, Shijiazhuang Hebei 050091, China;
    2Hebei Traditional Chinese Medicine Formula Granule Engineering & Technology Innovate Center, Shijiazhuang Hebei 050091, China;
    3Quality Evaluation & Standardization Hebei Province Engineering Research Center of Traditional Chinese Medicine, Shijiazhuang Hebei 050091, China
  • Received:2024-07-05 Online:2024-11-15 Published:2024-11-20

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摘要： 目的 建立超高效液相色谱串联三重四极杆质谱（UPLC-MS/MS）法测定蒲公英叶中15种成分含量的方法,结合多元统计分析为蒲公英叶药材质量评价提供参考。方法 采用Waters Acquity UPLC® HSS T3色谱柱,以乙腈-0.1%甲酸水为流动相,以多重反应监测（MRM）负离子模式检测,结合聚类分析和最小偏二乘判别分析进行评价。结果 15种化学成分在各自范围内线性关系良好（r≥0.997 7）,精密度和准确性良好,相对标准偏差（RSD）均<5%。聚类分析和OPLS-DA分析均可把样品分为4类,可将菊苣酸、单咖啡酰酒石酸、芦丁、秦皮乙素、金丝桃苷、异槲皮苷和木犀草苷作为蒲公英叶药材质量标志物的选择参考。结论 建立的方法准确性好,能够为蒲公英叶药材质量控制提供参考。

关键词: 蒲公英叶, 菊苣酸, 单咖啡酰酒石酸, 芦丁, 秦皮乙素, 超高效液相色谱串联三重四极杆质谱, 含量测定, 质量控制, 化学成分

Abstract: Objective To establish a UPLC-MS/MS method to determine the contents of 15 components in Dandelion leaves in order to provide a reference for quality evaluation. Methods The Waters Acquity UPLC® HSS T3 Column was used while acetonitrile-0.1% formic acid water served as the mobile phase. Negative electrospray ionization was performed in a multiple reaction monitoring (MRM) mode. Cluster analysis and least partial binary analysis were conducted for evaluation. Results The 15 components had a good linear relationship within their respective range (r≥0.997 7), with good precision and accuracy (RSD <5%). The samples could be divided into four categories by clustering and OPLS-DA analysis, and chichoric acid, cafftaric acid, rutin, esculetin, hyperoside, isoquercetin and cynaroside were used as reference for the selection of quality markers in Dandelion leaves. Conclusion This method is accurate enough to provide references for quality control of Dandelion leaves.

Key words: Dandelion Leaves, Chichoric Acid, Cafftaric Acid, Rutin, Esculetin, UPLC-MS/MS, Content Determination, Quality Control, Chemical Composition

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