Distribution of Scopolamine in Daturae Flos Based on SEM Technology and UPLC Chromatography

doi:10.19803/j.1672-8629.20241031

Abstract

Abstract: Objective To study the distribution of scopolamine in Daturae Flos via quantitative analysis combined with ultra-microscopic structural analysis. Methods The plant was divided into seven sections: the whole flower, lower segment, middle segment, upper segment, calyx, ovary and corolla. Scanning electron microscopy (SEM) was employed to observe and analyze the ultra-microscopic structures of the seven parts. Results SEM observation found that the secretory cells of Daturae Flos, such as glandular hairs, stomata and crystals, were concentrated in the ovary and its adjacent parts. The content of scopolamine was the highest in the ovary, followed by the lower segment, whole flower, upper segment, corolla, middle segment and calyx. The content of scopolamine varied across these parts. The corolla and calyx, which accounted for a large proportion of the volume, had lower contents than in the ovary. The large size and fragility of the decoction pieces of Daturae Flos made it difficult to maintain their integrity during transportation and storage. In addition, Daturae Flos was a toxic medicinal material. These properties could impact the clinical efficacy and safety of Daturae Flos. Conclusion This study reveals where the current quality evaluation system for Daturae Flos can be improved, and the research results are of referential value for ensuring the clinical efficacy and safety of medication of this medicinal material.

Key words: DATURAE FLOS, Scopolamine, UPLC, Ultra-Microscopic Structure, Electron Microscope, Distribution of Active Ingredients

CLC Number:

WANG Jizhou, XU Lu, ZHU Lili, XUE Shujuan, CHEN Suiqing, SUN Xiaoya. Distribution of Scopolamine in Daturae Flos Based on SEM Technology and UPLC Chromatography[J]. Chinese Journal of Pharmacovigilance, 2025, 22(11): 1236-1240.

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https://zgywjj.magtechjournal.com/EN/Y2025/V22/I11/1236

References

[1] Chinese Pharmacopoeia Commission.Pharmacopoeia of the People's Republic of China, Volume I(中华人民共和国药典一部)[M]. Beijing: China Medical Science Press, 2020: 279.
[2] ZHANG BP.The Effect and Mechanism of Flos Daturae Extract in the Treatment of Psoriasis through Anti-Inflammatory-Angiogenesis[D]. Beijing: Beijing University of Chinese Medicine, 2024.
[3] ZHU JL, DENG YJ, HE YS, et al.Research Progress on Chemical Constituents, Pharmacological Effect and Clinical Applications of Daturae Flos[J]. Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志), 2021, 27(23): 201-209.
[4] ZHOU HC.Pharmacological Function and Clinical Application Research of Flos Daturae[J]. Clinical Journal of Chinese Medicine(中医临床研究), 2017, 9(9): 129-130.
[5] LIU Y, RONG XH, TAN JY, et al.Study on the Chemical Components of Flavonoids and Alkaloids in the Leaves of Daturae Flos[J]. Chinese Traditional and Herbal Drugs(中草药), 2021, 52(14): 4141-4152.
[6] LIU Y, WANG SY, JIANG HB, et al.Research on the Chemical Comp-onents of Chrysanthemum Seeds[J]. Chinese Traditional Patent Medicine(中成药), 2021, 43(8): 2092-2100.
[7] LIU Y, WANG YF, YANG SH, et al.Separation and Identification of Chemical Components in Hibiscus Manihot[J]. World Chinese Medicine(世界中医药), 2022, 17(3): 359-364.
[8] GAN H, HE J, WANG XY, et al.Research on the Chemical Composition of the Golden Flower[J]. Chinese Pharmaceutical Journal(中国药学杂志), 2021, 56(2): 94-98.
[9] TAN XL.Yangjinhua Alkaloids Role of TGF-β1-induced EMT in Humanlung Adenocarcinoma Cell A549 Cells for Research[D]. Hangzhou: Zhejiang Chinese Medical University, 2014.
[10] JIANG YF, GAO JC, TIAN CH, et al.Literature Analysis of 165 Cases of Poisoning Adverse Events Induced by Datura metel L.[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2016, 13(4): 233-235, 239.
[11] XU L, YANG JF, SHEN ZR, et al.Ultramicrostructure of Leaves of Five Toxic Chinese Herbs Including Alangium chinense(Lour.) Harms[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2022,19(4): 361-368.
[12] XIA YY, LI Y, QIN KM, et al.Study on Processing Technology of Fried Daturae Flos by Orthogonal Experiment[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy(中国民族民间医药), 2021, 30(9): 58-62.
[13] ZHANG L, WANG Y.HPLC Fingerprint Analysis for Flos Daturae[J]. Chinese Journal of Information on Traditional Chinese Medicine(中国中医药信息杂志), 2008, 15(12): 53-54.
[14] LI D, WANG LY, MOU XF, et al.Quality Evaluation of Daturae Flos from Different Producing Areas and Effects of Extraction Methods on Extraction Yield of Scopolamine[J]. Journal of Yantai University(Natural Science and Engineering Edition)(烟台大学学报自然科学与工程版), 2019, 32(3): 246-249.
[15] KANG YG, JI JY, WANG JP, et al.A Survey and Pharmaceutical Prospects of Rabdosia Species[J]. China Journal of Chinese Materia Medica(中药通报), 1987(12): 12-15, 57.
[16] LIU Y, RONG XH, TAN JY, et al.Study on the Chemical Components and Anti-Inflammatory Activities of the Leaves of the Golden Flower[J]. Chinese Traditional Patent Medicine(中成药), 2022, 44(6): 1829-1839.
[17] WANG Y, HE J, WANG XY, et al.Research on Alkaloid Components in Hibiscus Manihot[J]. Chinese Traditional and Herbal Drugs(中草药), 2021, 52(20): 6163-6167.