Abstract: ObjectiveTo study the hepatotoxity mechanism of refined products from Bupleurum Saikosaponin by Alcohol Elution, and to discuss the relevance between hepatotoxcity and hepatic fibrosis, in order to provide the basis of hepatotoxity mechanism. MethodsRefined products from Bupleurum Saikosaponin by alcohol elution of different dosages(ig) were given to Wistar rats for fifteen days consecutively. The changes of hydroxyproline in serum and liver tissue were detected and the histopathological study was performed. ResultsOn the 15th day after ig the level of hydroxyproline in serum and liver tissue increased obviously, which is in line with the dosages increased. Masson staining shows the pathological changes of liver collagen fiber extension is obvious, and connected to each other surrounding the hepatic lobule, leading to destruction of the normal lobular architecture, false lobule. Compared with the control group, the degree of liver damage has certain dependence on the dose. ConclusionLong-term administration of the refined products from Bupleurum Saikosaponin by alcohol elution with large doses can result in liver fibrosis in rats; the results of this study provide some experimental basis for the safe use of Bupleurum inclinical .The total Bupleurum saikosaponin was the main material basis of hepatoxicity.

Key words: Bupleurum Saikosaponin, hepatotoxicity, hepaticfibrosis, hydroxyproline