Abstract: Objective To study the effects of Yi-qi Yang-yin San-jie herbal compound on cardiomyopathy of diabetic rats and the expression of AngⅡ of myocardial tissue. Methods The diabetic model was established by streptozotocin injection to the abdominal cavities of rats.The male SD rats were randomly divided into three groups: normal control group, diabetes mellitus group and herbal compound group. The rats of different groups were treated with relevamt medicinals respectively by intragastrical method. In the end of 4th and 8th weeks, the rats were sacrificed and their hearts is taken out, tissue slice is taken and the morphological changes were observed under optical microscope. Detect protein expression of AngⅡ through immunohistochemistry. AngⅡ mRNA level were detected by in situ hybridization method. Statistical image analysis Results . Results In the end of 4th and 8th weeks, as compared to the normal control group, levels of myocardial AngⅡ and AngⅡ mRNA expression in myocardium, were much higher in those of diabetes mellitus group, P<0.01. Compared with diabetes mellitus group, expression level of AngⅡ and AngⅡ mRNA in herbal compound group was much lower, <0.01. Conclusion Yi-qi Yang-yin San-jie herbal compound could downregulate expression of AngⅡ and had a therapeutical effect on cardiomyopathy in DM rats.

Key words: Yi-qi Yang-yin San-jie, diabetes, cardiomyopathy, renin-angiotensin system