Study on Liver and Kidney Toxicity of 4 Components of Polygoni Multifori Radix on Mice after Intragastric Administration for 14 Days

Abstract

Abstract: Objective To study the liver and kidney toxicity of stilbeneglycoside, rhein, physcion and emodin and provide useful exploration for the hepatotoxicity study of Polygoni Multifori Radix. Methods 50 ICR mice were randomly divided into control group, stilbeneglycoside group, rhein group, physcion group and emodin group. Intragastric administration was performed once daily with the dosage of 100 mg·kg-1 and the control group was given a corresponding amount of normal saline for 14 days. After the last delivery, foods were forbidden but the water was provided for 12 hours. The body weights were weighed and serum, liver and kidney were obtained to calculate the hepatic index and renal coefficient. Serum biochemical indicators and mRNA level of liver bile acid transporter were detected. Results The body weight of rhein, physcion and emodin groups were lower than the control group (P <0.05) and the hepatic index of rhein increased significantly (P <0.05). TG, CRE, ALB and GLU of stilbeneglycoside group increased markedly (P <0.05 or P <0.01). Rhein, physcion and emodin of anthraquinones made BUN dramatically decreased (P <0.05 or P <0.01), while rhein and emodin could induce TG decreased (P <0.05 or P <0.01). The 4 drugs had significant effects on bile acid transporters such as MRP2 and BSEP (P <0.05 or P <0.01). Conclusion Stilbeneglycoside, rhein, physcion and emodin of Polygoni Multifori Radix could change the serum biochemical indicators of ICR mice with the dosage of 100 mg·kg-1 for 14 days and have obvious effects on different bile acid transporters.

Key words: Polygoni Multifori Radix, liver injury, mice, cholestasis, bile acid transporter

CLC Number:

R965

SONG Lei, WANG Qin, BI Yanan, SHI Hong, ZHANG Yue, ZHOU Kun. Study on Liver and Kidney Toxicity of 4 Components of Polygoni Multifori Radix on Mice after Intragastric Administration for 14 Days[J]. Chinese Journal of Pharmacovigilance, 2018, 15(9): 513-517.

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