Effects of Tannin Part of Tibetan Medicine Phyllanthi Fructus on Apoptosis and Cell Cycle of Human Tumor Cells

Abstract

Abstract: Objective To investigate the mechanism of antitumor activity of tannin part of Tibetan medicine Phyllanthi Fructus on human tumor cells in vitro. Methods The human tumor A549 cells and BEL-7402 cells were treated with tannin part of Tibetan medicine Phyllanthi Fructus with 40, 80 and 160 μg·mL^-1 for 48 h. They were detected by MTT assay.Morphology changes in cells were examined under the inverted phase contrast microscope. Cell cycle and apoptosis in both cells were determined by flow cytometry. Results MTT assay showed a significant cytotoxicity on A549 cells and BEL-7402 cells in different concentration of tannin part of Tibetan medicine Phyllanthi Fructus for 48 h. Significant growth inhibition and cells shrinkage were observed in cells that received treatment. Significant cells early apoptosis and G2/M phase block were induced by tannin part of Tibetan medicine Phyllanthi Fructus. Conclusion Tannin part of Tibetan medicine Phyllanthi Fructus showed antitumor activity in vitro. Inducing tumor apoptosis and cell cycle block contribute to this activity.

Key words: tannin part of Phyllanthi Fructus, antitumor, apoptosis, cell cycle

CLC Number:

R916.696

CHEN Jing, FENG Guang-yuan, LI Deng-ke, SHI Lu-yuan, SUN Zhen-xiao, ZHANG Lan-zhen. Effects of Tannin Part of Tibetan Medicine Phyllanthi Fructus on Apoptosis and Cell Cycle of Human Tumor Cells [J]. Chinese Journal of Pharmacovigilance, 2016, 13(4): 193-196.

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