Abstract: ObjectiveTo study the time-toxicity relationship caused by different components of Radix Aconite Lateralis to mice. MethodsMice were grouped according to different time, to observe the death condition and toxicity of mice. The numbers of autonomic activities and rotarod test were observed and recorded. The changes of the activity of SOD, MDA, LDH and NSE were detected. ResultsThe changes of autonomic activities of mice with water extraction were strengthened and then weakened, the changes of LDH in serum were lifted marked at 30 minutes. The changes of autonomic activities and standing time of mice with alcohol extraction were weakened and then strengthened, significant reduction was at 60 minutes, the changes of SOD in serum were reducing marked from 15 to 120 minutes. The changes of autonomic activities and standing time of mice with whole components were reduced at 90 minutes, the changes of SOD in serum were reducing. The levels of neuron specific enolization enzyme(NSE) from the three groups' mice serum were changed to rise in different degree, and alcohol extraction > water extraction > the whole components. ConclusionSingle intragastric administrations of different components of Radix Aconite Lateralis with certain dosage may induce acute nerve injury in mice and show certain time-toxicity relationship. The study on damage mechanism and the change of behavioral science, neurotransmitter levels and neuron would be discussed in the following study.

Key words: Radix Aconite Lateralis, different components, neurotoxicity, time-toxicity relationship