Determination of Six Kinds of Aconitum Alkaloids in Aconitum Carmichaeli and Its Samples by HPLC

Abstract

Abstract: ObjectiveTo establish a HPLC method for the determination of six kinds of Aconitum alkaloids in Aconitum carmichaeli and its samples. MethodsA C18 column was used, and acetonitrile- tetrahydrofuran(25:15) as the mobile phase A, 0.1mol·L-1 NH4AC solution as the mobile phase B, gradient elute with a flow rate of 1.0mL·min-1 and a detection wavelength of 235nm. ResultsThe six kinds of Aconitum alkaloids were separated, and the calibration curves of them were in good linearity over the certain range respectively. ConclusionThis method is simple and accurate. It can be used in the identification and quality control of A. carmichaeli.

Key words: Aconitum carmichaeli, Aconitum alkaloid, HPLC

CLC Number:

R917

LUAN Yong-fu, SUN Rong. Determination of Six Kinds of Aconitum Alkaloids in Aconitum Carmichaeli and Its Samples by HPLC[J]. Chinese Journal of Pharmacovigilance, 2014, 11(5): 271-274.

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Determination of Six Kinds of Aconitum Alkaloids in Aconitum Carmichaeli and Its Samples by HPLC

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