Abstract: Objective To establish a HPLC method for the simultaneous determination of Oleanolic Acid and Ursolic Acid in Medofenoxate fruits. Methods Agilent XDBC18(4.6 mm×250 mm,5μm) column was applied, using methanolacetonitrile-0.3% phosphoric acid(30 ：60 ：10) as mobile phase with the rate of 0.8mL·min^-1. The column temperature was room temperature and the detective wavelength was 210nm. Results A good linearity was obtained over the range of 0.2052～3.2832μg for Oleanolic Acid(r=0.9995). The average recovery(n=6) was 101.11%(RSD=1.69%);A good linearity was obtained over the range of 0.2072～3.3152μg for Ursolic Acid(r=0.9993). The average recovery(n=6) was 98.51%(RSD=2.67%). Conclusion The method is practical, convenient, accurate, and can be used as the simultaneous determination of Oleanolic Acid and Ursolic Acid in Medofenoxate fruits.

Key words: medofenoxate fruit, oleanolic acid, ursolic acid, HPLC