The Mechanism of Bazhen Decoction in Treating Immune Thrombocytopenia Based on Network Pharmacology

doi:10.19803/j.1672-8629.2021.10.07

Abstract

Abstract: Objective To explore the mechanism by which Bazhen Decoction combats immune thrombocytopenia (ITP) using network pharmacology. Methods The main active ingredients of Bazhen Decoction were screened using the pharmacological database and analysis platform (TCMSP) of the Chinese Medicine System. Drugbank database was searched for target prediction. Cytoscape 3.7.1 software was used to build a chemical composition-target network. Online Mendelian Inheritance In Man (OMIM), human gene database (Gene Cards), and Drugbank were searched for the related target genes of ITP.The target of active ingredients of the drug was mapped to the ITP target to obtain the intersection target, which was the predicted target for ITP of Bazhen Decoration. The STRING database was used to construct an online intersection target protein interaction network (PPI) to screen out key target genes. Gene ontology (GO) analysis and enrichment analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to analyze intersection targets using DAVID 6.8 and online tools. Results A total of 160 chemical components, 247 potential targets, 304 ITP-related targets, and 20 common targets were found in Bazhen Decoration. Quercetin, kaempferol, and 7-Methoxy-2-methyl isoflavone regulated most of the targets associated with ITP. GO biological function analysis showed that this decoration was involved in such biological processes as protein phosphorylation, apoptosis, signal transduction, and proliferation. The enrichment results of KEGG pathway showed that the potential pathways through which Bazhen Decoration could treat ITP included PI3K-Akt signaling pathway, Ras signaling pathway, insulin signaling pathway, FoxO signaling pathway, MAPK signaling pathway, Rap1 signaling pathway, and T cell receptor signaling pathway. Conclusion Bazhen Decoction can combat ITP through the complex mechanism of multiple components, multiple targets and multiple pathways. The core targets are RAF1, PIK3CG, IKBKB, MAPK10, EGFR, GSK3B, and MAPK14. The regulatory mechanism mainly involves immune regulation, signal transduction and proteoglycan regulation in related signaling pathways.

Key words: Bazhen Decoction, immune thrombocytopenia (ITP), network pharmacology, mechanism

CLC Number:

R973R932

HUANG Wei, LUO Yaqin, WANG Xiao. The Mechanism of Bazhen Decoction in Treating Immune Thrombocytopenia Based on Network Pharmacology[J]. Chinese Journal of Pharmacovigilance, 2021, 18(10): 930-935.

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