中国药物警戒 ›› 2017, Vol. 14 ›› Issue (12): 727-729.

• 常用中药减毒研究专栏 • 上一篇    下一篇

柴胡醋制前后水提组分对L02细胞影响研究

赵新妹1,2,张志一3,李晓宇1,2, 孙蓉4*   

  1. 1山东省中医药研究院，山东 济南 250014；
    2山东中医药大学，山东 济南 250355；
    3山东新时代药业有限公司，山东 临沂 273400；
    4山东大学齐鲁医学院，山东 济南 250012
  • 收稿日期:2017-12-11 修回日期:2018-01-19 出版日期:2017-12-20 发布日期:2018-01-19
  • 通讯作者: 孙蓉，女，教授·博导，中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:赵新妹，女，在读硕士，中药药理与毒理。
  • 基金资助:
    国家自然科学基金项目（81374059）：柴胡肝毒性与功效、证候关系的基础研究；山东省重点研发计划项目（2016GSF202039）：基于蛋白组学差异表达和血清谱-效-毒关联评价的醋柴胡功效与安全性研究；山东省重点研发计划（2016ZDJS07A21）：柴胡类经方精准化研究与评价关键技术及二次开发；山东省泰山学者工程专项经费资助（ts201511107）。

Effects of Water Extracts from Bupleuri Radix and Vinegar-baked Bupleuri Radix on L02 Cells in vitro

ZHAO Xin-mei1,2, ZHANG Zhi-yi3 ,LI Xiao-yu1,2 ,SUN Rong4*   

  1. 1Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    2Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
    3Shandong Newtimes Pharmaceutical Co. Ltd., Shandong Linyi 273400, China;
    4Cheeloo College of Medicine, Shandong University, Shandong Jinan 250012, China
  • Received:2017-12-11 Revised:2018-01-19 Online:2017-12-20 Published:2018-01-19

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摘要： 目的 研究柴胡醋制前后水提组分对L02细胞的增殖作用。方法 采用CCK8法分别检测柴胡与醋柴胡水提组分对L02细胞生长的影响。采用生化法测定细胞上清液中谷丙转氨酶（ALT）、谷草转氨酶（AST）和乳酸脱氢酶（LDH）的含量。结果 CCK8法显示柴胡与醋柴胡水提组分均能抑制L02细胞的生长增殖，且细胞抑制作用与剂量呈正相关，与正常组比较作用显著，且作用强度为柴胡水提组分>醋柴胡水提组分。柴胡水提组分与醋柴胡水提组分作用于细胞24 h后，半数抑制浓度（IC50）分别为1 173.87和1 205.59 mg·L-1；生化法测定结果显示ALT、AST与LDH含量升高。结论 柴胡醋制前后水提组分均对L02细胞产生毒性作用，且毒性作用强度柴胡水提组分>醋柴胡水提组分。

关键词: 柴胡, 醋柴胡, 毒性, L02细胞

Abstract: Objective To compare the effects of the water extracts from Bupleuri Radix and vinegar-baked Bupleuri Radix on L02 cells in vitro. Methods The effect of the water extracts from Bupleuri Radix and vinegar-baked Bupleuri Radix on the growth of L02 cells were tested by CCK8 method. ALT, AST and LDH in the culture supernatant were determined by biochemical method. Results CCK8 method showed that the water extracts of Bupleuri Radix and vinegar-baked Bupleuri Radix could inhibit the proliferation of L02 cells, and the cell viability was positively correlated with the dose which compared with the normal group. The cell inhibitory effect strength is the water extract of Bupleuri Radix > the water extract of the vinegar-baked Bupleuri Radix. IC50 of the water extract from Bupleuri Radix and vinegar-baked Bupleuri Radix which had acted 24 h, is respectively 1 173.87 and 1 205.59 mg·L-1. The results of biochemical assay showed that the content of ALT, AST and LDH increased. Conclusions The water extract of Bupleuri Radix and vinegar-baked Bupleuri Radix all have toxic effects on L02 cells, and the inhibitory effect of the water extract from Bupleuri Radix on the growth of L02 cells was stronger than that of the water extracts from vinegar-baked Bupleuri Radix.

Key words: Bupleuri Radix, vinegar-baked Bupleuri Radix, toxicity, L02 cells

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