中国药物警戒 ›› 2016, Vol. 13 ›› Issue (5): 257-259.

• 基础与临床研究 •    下一篇

汉防己甲素对MCF-7 细胞Akt激酶活性影响的研究

武建毅,沈清,金赟洁,姚晓祥,朱纪华,朱麟玉,丁昭珩,黄晓东   

  1. 上海市普陀区人民医院，上海 200060
  • 收稿日期:2016-03-07 修回日期:2016-06-30 出版日期:2016-05-20 发布日期:2016-06-30
  • 通讯作者: 黄晓东，男，副主任医师，呼吸道/消化道恶性肿瘤治疗。E-mail:huangxiaodong@sina.com
  • 作者简介:武建毅，男，硕士，主治医师，肿瘤生物治疗。

Study on Effect of Tetrandrine on Akt Activity in MCF-7 Cell Line

WU Jian-yi, SHEN Qing, JIN Yun-jie, YAO Xiao-xiang, ZHU Ji-hua, ZHU Lin-yu, DING Zhao-heng, HUANG Xiao-dong   

  1. Department of Oncology, Putuo District People’s Hospital of Shanghai, Shanghai 200060, China
  • Received:2016-03-07 Revised:2016-06-30 Online:2016-05-20 Published:2016-06-30

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摘要： 目的 探讨汉防己甲素诱导人乳腺癌细胞MCF-7凋亡的分子机制。方法 采用流式细胞法测定不同浓度汉防己甲素（1、5、10 μmol·L-1）诱导MCF-7凋亡率；免疫印迹法检测汉防己甲素对MCF-7细胞总Akt及p-Akt ser437、p-Akt Thr308表达的影响；Akt激酶活性检测试剂盒检测Akt激酶活性的改变。结果 不同浓度的Tet（1、5、10 μmol·L-1）对培养的乳腺癌细胞MCF-7可有效的诱导凋亡；同时Tet可以有效的抑制p-Akt ser437、p-Akt Thr308蛋白表达，但对总Akt蛋白的表达没有影响。Akt 激酶活性的分析显示：不同浓度的Tet处理MCF-7细胞48 h后，磷酸化的GSK3α/β蛋白浓度逐渐下降，间接提示Akt激酶活性下降。结论 汉防己碱可能通过抑制Akt激酶活性并影响其下游的信号途径诱导乳腺癌细胞凋亡。

关键词: 汉防己甲素, 乳腺肿瘤, Akt激酶

Abstract: Objective To study the cell signal mechanism of tetrandrine-induced apoptosis of human breast cancer cell line MCF-7. Methods The effects of tetrandrine-induced apoptosis of MCF-7 (1, 5, 10 μmol·L-1) was detected by flow cytometry. Western blot was performed to assess expression of Akt, p-Akt ser437 and p-Akt Thr308 in MCF-7. The activity of Akt was detected by Akt activity assay kit (KinaseSTAR). Results Different doses of tetrandrine (Tet) could induce apoptosis of MCF-7 in a dose-dependent manor. Tet could significantly inhibit expression of p-Akt ser437and p-Akt Thr308, but had no effect to expression of total Akt in MCF-7. Tet could down-regulate the activity of Akt of MCF-7. Conclusion Tet can induce apoptosis of MCF-7 cells through suppressing Akt activity.

Key words: tetrandrine, breast cancer, Akt

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