中国药物警戒 ›› 2012, Vol. 9 ›› Issue (1): 1-2.

• 基础及临床研究 •    下一篇

高效液相色谱法测定首乌丸中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量

成旭东，孙芸，刘扬*   

  1. 苏州大学药学院，江苏 苏州 2152123
  • 收稿日期:2011-09-07 出版日期:2012-01-10 发布日期:2015-08-10
  • 通讯作者: 刘扬,博士,讲师,药物新技术与新剂型。E-mail:liuyang@suda.edu.cn
  • 作者简介:成旭东，男，硕士，工程师。

Determination of 2,3,5,4'- Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu Pills by HPLC

CHENG Xu-dong, SUN Yun, LIU Yang*   

  1. College of Pharmaceutical Science, Soochow University, Jiangsu Suzhou 215123, China
  • Received:2011-09-07 Online:2012-01-10 Published:2015-08-10

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摘要： 目的高效液相法测定测定首乌丸中2，3，5，4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷成分含量;方法 采用RP-HPLC，色谱柱Ultimate XB-C18（4.6mm×250mm，5μm）;流动相为乙腈-水（25:75）;检测波长：320nm;流速：1mL·min-1;柱温30℃。结果3批首乌丸中2，3，5，4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量为3.895~4.136 mg·g-1;结论 该法稳定、简便，适用于首乌丸制剂质量控制。

关键词: 首乌丸, 2, 3, 5, 4'-四羟基二苯乙烯-2-O-β, -D-葡萄糖苷, 高效液相色谱法

Abstract: Objective Determine of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills. The method of determine 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills was established. MethodsThe samples were separated at 30℃ on a Ultimate XB-C18 column(4.6mm×250 mm, 5μm). When 2,3,5,4'-Tetrah-ydroxystilbene-2-O-β-D-glucoside was eluted with acetonitrile and water(25:75). The detection wavelength was 320nm.The flow rate was all 1ml·min-1. ResultsThe content of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills was 3.895~4.136mg·g-1. ConclusionThe method was stable and convenient. This method was suitable for the quality control of Shouwu pills.

Key words: Shouwu pills, 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-β, -D-glucoside, HPLC