中国药物警戒 ›› 2025, Vol. 22 ›› Issue (2): 139-146.
DOI: 10.19803/j.1672-8629.20230660

• 雷公藤肝损伤相关研究专栏 • 上一篇    下一篇

雷公藤甲素对原代肾上腺皮质细胞类固醇激素分泌的影响

王红丽1, 杜娟2, 曹梦琪1, 张陆勇1,3, 江振洲1,4#, 黄鑫1,4,*   

  1. 1中国药科大学新药筛选与药效评价中心,江苏 南京 210009;
    2重庆轻工职业学院药学与护理学院,重庆 400065;
    3广东药科大学新药研发中心,广东 广州 510006;
    4江苏省药效研究与评价服务中心,江苏 南京 210009
  • 收稿日期:2023-10-24 出版日期:2025-02-17 发布日期:2025-02-17
  • 通讯作者: *黄鑫,女,副研究员·硕导,体内药物分析与中药毒效相关性研究。E-mail: huangxin@cpu.edu.cn;#为共同通信作者。
  • 作者简介:王红丽,女,在读硕士,药物分析学。
  • 基金资助:
    国家自然科学基金资助项目（82174072）

Effects of Triptolide on Secretion of Steroid Hormone in Primary Adrenocortical Cells

WANG Hongli1, DU Juan2, CAO Mengqi1, ZHANG Luyong1,3, JIANG Zhenzhou1,4#, HUANG Xin1,4,*   

  1. 1New Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing Jiangsu 210009, China;
    2School of Pharmacy and Nursing, Chongqing Vocational College of Light Industry, Chonqing 400065, China;
    3Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou Guangdong 510006, China;
    4Jiangsu Center for Pharmacodynamics Research and Evaluation, Nanjing Jiangsu 210009, China
  • Received:2023-10-24 Online:2025-02-17 Published:2025-02-17

PDF (PC)

50

摘要： 目的 建立Wistar大鼠原代肾上腺皮质细胞的提取验证方法并探究雷公藤甲素对原代肾上腺皮质细胞的影响与机制。方法 提取Wistar大鼠原代肾上腺皮质细胞,验证其血清依赖性和类固醇激素分泌功能;考察雷公藤甲素对原代肾上腺皮质细胞的毒性及周龄变化对毒性敏感性的影响,通过PCR和WB实验初步探究其机制。结果 离体培养72 h的原代肾上腺皮质细胞具有血清依赖性和类固醇激素分泌功能。大鼠周龄影响毒性敏感性,雷公藤甲素剂量依赖性抑制类固醇激素分泌,抑制3β-羟基类固醇脱氢酶2（HSD3B2）的基因和蛋白表达。结论 雌性Wistar大鼠原代肾上腺皮质细胞是探究药物对肾上腺类固醇激素分泌影响的良好模型;雷公藤甲素抑制原代肾上腺皮质细胞类固醇激素分泌与HSD3B2的基因和蛋白表达降低有关。

关键词: 雷公藤, 雷公藤甲素, 肾上腺, 原代肾上腺皮质细胞, 皮质酮, 孕酮, 3β-羟基类固醇脱氢酶2, 雌性Wistar大鼠

Abstract: Objective To establish a method for the extraction and verification of primary rat adrenocortical cells and to investigate the effect of triptolide on primary rat adrenocortical cells and the mechanism. Methods Primary adrenocortical cells were isolated and cultured in culture plates, their serum dependence and steroid hormone secretion function were verified, the toxicity of triptolide on primary adrenocortical cells and the effect of age on toxicity sensitivity to triptolide were investigated, and the mechanism was explored via PCR and WB experiments. Results The primary rat adrenocortical cells were serum-dependent and could secrete steroid hormone after 72 h in vitro. The toxicity sensitivity of triptolide was influenced by week age. Triptolide dose-dependently inhibited steroid hormone secretion and inhibited HSD3B2 gene and protein expressions. Conclusion The primary adrenocortical cells of female Wistar rats can serve as a good model to study the effect of drugs on adrenal steroid hormone secretion, whose inhibition by triptolide in primary adrenocortical cells is associated with decreased gene and protein expressions of HSD3B2.

Key words: Tripterygium wilfordii Hook.f., Triptolide, Adrenal Gland, Primary Adrenocortical Cells, Corticosterone, Progesterone, HSD3B2, Female Wistar Rat

中图分类号: