中国药物警戒 ›› 2025, Vol. 22 ›› Issue (8): 851-855.
DOI: 10.19803/j.1672-8629.20250010

• 中药抗病毒与解毒机制专栏 • 上一篇    下一篇

一叶抗流感胶囊对人冠状病毒229E感染致小鼠肺炎的影响

王馨蔚1, 彭一峰2△, 孙静1, 冀祖恩2, 包蕾1, 罗恒磊2, 耿子涵1, 张虎娟2, 李舒冉1, 张敬升1, 郭姗姗1, 崔晓兰1#, 赵荣华1*   

  1. 1中国中医科学院中药研究所,北京 100700;
    2新疆甘草及制品研究重点实验室,新疆 库尔勒 841011
  • 收稿日期:2025-01-04 出版日期:2025-08-15 发布日期:2025-08-13
  • 通讯作者: *赵荣华,女,博士,副研究员,中药药效作用机制研究。E-mail: zhaoronghua203@163.com #为共同通信作者。
  • 作者简介:王馨蔚,女,在读硕士,中药抗感染药理研究。△为并列第一作者。
  • 基金资助:
    中国中医科学院科技创新工程（C12021A04606）; 新疆维吾尔自治区重大科技专项项目（2022A03007-1）; 国家自然科学基金资助项目（82305078）

Effect of Yiye Anti-Influenza Capsules on Pneumonia Induced by Human Coronavirus 229E Infection in Mice

WANG Xinwei1, PENG Yifeng2△, SUN Jing1, JI Zuen2, BAO Lei1, LUO Henglei2, GENG Zihan1, ZHANG Hujuan2, LI Shuran1, ZHANG Jingsheng1, GUO Shanshan1, CUI Xiaolan1#, ZHAO Ronghua1*   

  1. 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    2Xinjiang Key Laboratory for Research of Licorice and Products, Korla Xinjiang 841000, China
  • Received:2025-01-04 Online:2025-08-15 Published:2025-08-13

PDF (PC)

7

摘要： 目的 探究一叶抗流感胶囊（YYKLG）对人冠状病毒229E（HCoV-229E）感染所致小鼠肺炎的治疗作用。方法 采用HCoV-229E滴鼻感染建模,小鼠根据体质量随机分为正常组,模型组,磷酸氯喹组,连花清瘟颗粒组,YYKLG高、中、低3个剂量组（分别为0.70 、0.35、0.18 g·kg-1·d-1）,共7组。感染当天开始正常组及模型组灌胃给予蒸馏水处理,其余组灌胃给药处理,共4 d,第5天对各组小鼠称重、解剖,评估YYKLG在高、中、低3个不同剂量下对小鼠肺指数、病毒载量、肺内细支气管及肺组织病理变化的影响。结果 YYKLG高、中剂量组小鼠肺指数明显下降;YYKLG高、中、低3个剂量组肺组织病毒载量显著下降。中药给药组与模型组相比均可不同程度减轻小鼠肺部细支气管和肺组织病理损伤,其中高、中剂量组的病变程度与模型组的病变程度比较,差异具有统计学意义。结论 YYKLG可以改善HCoV-229E病毒感染所致的模型小鼠肺炎症状,以期为中药治疗呼吸道疾病的临床应用提供参考。

关键词: 一叶抗流感胶囊, 人冠状病毒229E, 肺指数, 肺指数抑制率, 病毒载量, 病理变化, 小鼠

Abstract: Objective To investigate the therapeutic effect of Yiye anti-influenza capsules (YYKLG) against pneumonia in mice infected with human coronavirus 229E (HCoV-229E). Methods A mouse model of pneumonia was established via intranasal infection with HCoV-229E. The mice were randomly divided into seven groups, including high-dose, medium-dose, and low-dose YYKLG groups (0.70 , 0.35 and 0.18 g·kg-1·d-1, respectively). The effects of YYKLG at three doses on the lung index, viral load, pulmonary bronchioles and pathological changes of lung tissues in mice were assessed. Results The lung index of mice in the high and medium dose groups of YYKLG decreased significantly, so did the viral load of lung tissues in the three dose groups. Compared with the model control group, YYKLG reduced the pathological damage to lung bronchioles and lung tissues to varied extents, and the degree of pathological damage in the high and middle dose groups was significantly different from that of the model control group. Conclusion YYKLG can improve the symptoms of pneumonia in model mice infected with the HCoV-229E virus. This study is expected to provide a reference for the treatment of respiratory diseases with traditional Chinese medicine.

Key words: Yiye Anti-Influenza Capsule, HCoV-229E, Lung Index, Lung Index Inhibition Rate, Viral Load, Pathological Changes, Mice

中图分类号: