中国药物警戒 ›› 2012, Vol. 9 ›› Issue (1): 3-4.

• 基础及临床研究 • 上一篇    下一篇

HPLC法同时测定胆宁片中5种蒽醌类衍生物含量

张秋蓉1，王丽芳2，刘文燕2   

  1. 1 大理学院临床医学院，云南 大理 671000；
    2 大理学院药学院，云南 大理 67100
  • 收稿日期:2011-06-24 出版日期:2012-01-10 发布日期:2015-08-10
  • 作者简介:张秋蓉，女，硕士，讲师，内科学相关工作研究。

HPLC Simultaneous Determination of Five Kinds of Anthraquinones in Danning Tablets

ZHANG Qiu-rong1, WANG Li-fang2, LIU Wen-yan2   

  1. 1 Clinical Medicine, Dali University, Yunnan Dali 671000, China;
    2 Department of Pharmacy, Dali University, Yunnan Dali 671000, China
  • Received:2011-06-24 Online:2012-01-10 Published:2015-08-10

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摘要： 目的建立高效液相色谱法同时测定胆宁片中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚5个成分含量。方法采用Agela Venusil XBP-C18（4.6 mm×250 mm，5µm）色谱柱;流动相为乙腈（A）-0.1 %磷酸（B），进行梯度洗脱;流速1.0 mL·min-1;检测波长为226 nm。结果芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚进样量分别在0.040~0.800µg、0.040~0.800µg、0.120~2.400µg、0.040~0.800µg、0.040~0.800µg范围内线性关系良好。平均回收率（n=6）分别为96.85%、97.48%、98.69%、96.27%、95.68%，RSD分别为2.28％、1.72%、1.28%、1.64%、2.62%。结论该方法准确、可靠、重复性好，可作为该制剂的定量分析方法。

关键词: 胆宁片, 高效液相色谱法, 蒽醌, 含量测定

Abstract: Objective To develop an HPLC method for determining the contents of aloe-emodin、rhein、emodin、chrysophanol and physcione in Danning tablets. MethodsAgela Venusil XBP-C18(4.6 mm×250 mm, 5 μm) with acetonitrile(A) and 0.1 % phosphoric acid(B) as mobile phase by gradient elution, The flow rate was 1.0 mL·min-1 and the detection wavelength was 226 nm. ResultsThe injection volume of aloe-emodinrhein、emodin、chrysophanol and physcione were 0.040-0.800, 0.040-0.800, 0.120-2.400, 0.040-0.800, 0.040-0.800 μg respectively, The average recovery rates were(n=6) 96.85%(RSD=2.28%)、97.48%(RSD=1.72%)、98.69%(RSD=1.28%)、96.27%(RSD=1.64%)、95.68 %(RSD=2.62 %) respectively. ConclusionThe method was accurate, reliable, good repeatability and can be used as the determination analyse of the preparation.

Key words: danning tablets, HPLC, anthraquinone, determine content