Abstract: Objective Explore the effect of extracting methods on "quality-toxicity" comprehensive evaluation model by comparing the content of alkaloid and acute toxicity from Evodia Rutaecarpa(Juss.) Benth extracted by different methods. MethodsHPLC was used to determine the content of Evodiamine and Rutaecarpine in water extract and alcohol extract of Evodia Rutaecarpa(Juss.) Benth; the classical method of acute toxicity was used to determine the acute toxicity of Evodia rutaecarpa(Juss.) Benth. at different extraction, and compare the acute toxicity. ResultsThe content of Evodiamine and Rutaecarpine which included water extract and ethanol extract was separately 0.028% and 0.027%,0.046% and 0.075%. The two groups are unable to make LD₅₀, MTD results calculated in accordance with crude drug content were respectively 80.0 g·kg-1·d^-1 and 70.6g·kg^-1·d^-1. ConclusionThe content of Evodiamine and Rutaecarpine and acute toxicity of the Fructus Evodiae Rutaecarpae by different extracting methods were different, and death occurred in each sample, the MLD showed that the different extracts which extracted from the Fructus Evodiae Rutaecarpae at different solvents extraction had certainly toxicity in mice. The acute toxicity of different extracts on mice is: alcohol extraction group>water extraction group, the content of Evodiamine and Rutaecarpine in different extracts is: alcohol extraction group>water extraction group. Suggesting that it was certain effect of extracting methods on "quality toxicity" comprehensive evaluation model. It was more comprehensive, Objective and reasonable to determine extraction methods of toxic Chinese medicine through the comprehensive evaluation model.

Key words: Evodia Rutaecarpa(Juss) Benth., evaluation model, quality, toxicity, comprehensive evaluation