Abstract: Objective Explore the effect of extracting methods on "quality toxicity" comprehensive evaluation model by comparing the content of alkaloid and acute toxicity from Brucea javanica(L.)Merr. extracted by different methods. MethodsGC was used to determine the content of oleic acid in water extract and alcohol extract of Brucea javanica(L.)Merr.; the classical method of acute toxicity was used to determine the acute toxicity of Brucea javanica(L.)Merr. at different extraction, and compare the acute toxicity. ResultsThe content of oleic acid which included water extract and ethanol extract was separately1.83%、10.35%. The LD50 of water extract and alcohol extract of Fructus Brucea to ice were 4.023g·kg-1, 3.320g·kg-1. Diarrhea and cyanosis are main acute toxic symptoms. ConclusionThe content of oleic acid and acute toxicity of the Fructus Brucea by different extracting methods were different, and death occurred in each sample, the LD50 showed that the different extracts which extracted from the Fructus Brucea at different extracting methods had certainly toxicity in mice. The acute toxicity of different extracts on mice is: alcohol extraction group>water extraction group, the content of oleic acid in different extracts is: alcohol extraction group>water extraction group. Suggesting that it was certain effect of extracting methods on "quality toxicity" comprehensive evaluation model. It was more comprehensive, Objective and reasonable to determine extraction methods of toxic Chinese medicine through the comprehensive evaluation model.

Key words: Fructus Bruce, evaluation model, quality, toxicity, comprehensive evaluation