Abstract: Objective By comparing the volatile oil, total flavonoids content and the acute toxicity of different extracts in Folium Artemisiae Argyi, to explore the effect of different extraction solvents on the quality and toxicity of Folium Artemisiae Argyi. MethodsBy using pharmacopeial determination of volatile oil method, UV and the classic acute toxicity experiment method, to determine the content of volatile oil and total flavonoids, as well as compare the acute toxicity of different extracts. ResultsThe content of volatile oil is 0.76%, while the total flavonoids content of different extracts from the biggest to the smallest is alcohol extraction components, water extraction components and all components. The acute toxicity of different extracts from the biggest to the smallest is volatile oil, water extraction components, alcohol extraction components and all components. The LD₅₀ of volatile oil and water extraction components in Folium Artemisiae Argyi are 1.673 mL·kg^-1 and 80.228 g·kg^-1 while the 95% confidence limit are 1.5538 ~1.7954mL·