Abstract: Objective To study the "dosage-time-toxicity" relationship of hepatotoxicity induced by multiple intragastric administration of water extracted and alcohol extracted components of Cortex Periplocae to mice. Methods Mice are grouped according to different time or dose points, the general condition and toxic effects of mice were observed.The changes of the activity of ALT, AST, etc and liver, kidney index were detected. Results On the first day after administration the hepatotoxicity which displayed with obvious increase of activity in serum ALT, AST, especially the changes of AST were more sensitive. The conditions continued for about 7 days. Compared with the normal group, within 7 days after the administration, water extracted components in(0.78~15.0) g·kg-1 doses of scope, alcohol extracted components in(0.78~6.0) g·kg-1 doses of scope, they all cause significant damage to liver, the activity of ALT, AST, ALP, and TBI elevated, while ALB reduced, and liver ratio increased, it shows there were obvious "dosagetime-toxicity" relations. The degree of hepatotoxicity damage caused by the alcohol extracted composition is more serious than that by the water extracted composition. Conclusion Multiple intragastric administrations of water extracted and alcohol extracted components of Cortex Periplocae with certain dosage may induce acute hepatotoxical injury in mice and show an obvious "dosage-time-toxicity" relationship.

Key words: Cortex Periplocae water extracted and alcohol extracted components, mice, hepatotoxicity "dosage-timetoxicity"relationship