Abstract: Objective Determine of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills. The method of determine 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills was established. MethodsThe samples were separated at 30℃ on a Ultimate XB-C18 column(4.6mm×250 mm, 5μm). When 2,3,5,4'-Tetrah-ydroxystilbene-2-O-β-D-glucoside was eluted with acetonitrile and water(25:75). The detection wavelength was 320nm.The flow rate was all 1ml·min-1. ResultsThe content of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills was 3.895~4.136mg·g^-1. ConclusionThe method was stable and convenient. This method was suitable for the quality control of Shouwu pills.

Key words: Shouwu pills, 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-β, -D-glucoside, HPLC