Abstract: Objective To develop an HPLC method for determining the contents of aloe-emodin、rhein、emodin、chrysophanol and physcione in Danning tablets. MethodsAgela Venusil XBP-C18(4.6 mm×250 mm, 5 μm) with acetonitrile(A) and 0.1 % phosphoric acid(B) as mobile phase by gradient elution, The flow rate was 1.0 mL·min-1 and the detection wavelength was 226 nm. ResultsThe injection volume of aloe-emodinrhein、emodin、chrysophanol and physcione were 0.040-0.800, 0.040-0.800, 0.120-2.400, 0.040-0.800, 0.040-0.800 μg respectively, The average recovery rates were(n=6) 96.85%(RSD=2.28%)、97.48%(RSD=1.72%)、98.69%(RSD=1.28%)、96.27%(RSD=1.64%)、95.68 %(RSD=2.62 %) respectively. ConclusionThe method was accurate, reliable, good repeatability and can be used as the determination analyse of the preparation.

Key words: danning tablets, HPLC, anthraquinone, determine content