Chinese Journal of Pharmacovigilance ›› 2012, Vol. 9 ›› Issue (1): 1-2.

• Orginal Article •     Next Articles

Determination of 2,3,5,4'- Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu Pills by HPLC

CHENG Xu-dong, SUN Yun, LIU Yang*   

  1. College of Pharmaceutical Science, Soochow University, Jiangsu Suzhou 215123, China
  • Received:2011-09-07 Online:2012-01-10 Published:2015-08-10

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Abstract: Objective Determine of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills. The method of determine 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills was established. MethodsThe samples were separated at 30℃ on a Ultimate XB-C18 column(4.6mm×250 mm, 5μm). When 2,3,5,4'-Tetrah-ydroxystilbene-2-O-β-D-glucoside was eluted with acetonitrile and water(25:75). The detection wavelength was 320nm.The flow rate was all 1ml·min-1. ResultsThe content of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills was 3.895~4.136mg·g-1. ConclusionThe method was stable and convenient. This method was suitable for the quality control of Shouwu pills.

Key words: Shouwu pills, 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-β, -D-glucoside, HPLC