Applications of “Target Fishing” Technique in Pharmacological Research of Traditional Chinese Medicine

doi:10.19803/j.1672-8629.20240700

Abstract

Abstract: Objective To review the recent applications of “target fishing” technique in the fields of Traditional Chinese Medicine (TCM) in recent years in order to contribute to overcoming the bottleneck of TCM modernization caused by the complexity of TCM components and the uncertainty of target components. Methods Literature on “target fishing” techniques in the field of TCM in the past 15 years was searched. Research methods involved in the commonly used “target fishing” techniques were summarized. The strengths and weaknesses of these techniques in revealing the action or toxicity targets of TCM components were analyzed. Results “Target fishing” techniques applied in the field of TCM were mostly based on “affinity fishing”, which was often used in conjunction with liquid chromatography, mass spectrometry and other technologies for direct target research of TCM potent or toxic components on the basis of a clear mechanism of affinity fishing and toxicity. It was difficult to apply this technique to small molecules with close conformational relationships or to perform rapid and unbiased protein target identification for complex TCM systems. The interpretation of TCM theories in existing studies remained weak, and there was a lack of in-depth integration with TCM theories in subsequent validation studies or in-depth excavation. Conclusion The “target fishing” technique offers novel insights and methodologies for elucidating the molecular-level mechanisms of TCM action. This study proposes a research strategy for investigating the effects and toxicity of TCM, which should be thoroughly explored within the framework of TCM theories and substantiated from comprehensive system-level perspectives. The“target fishing” technique provides references for integrated evaluation of the “effect-toxicity” of TCM.

Key words: “Target Fishing” Technique, Identification of Target, Pharmacology of TCM, Toxicology of TCM, Efficacy-Toxicity Components

CLC Number:

R285
R994.11

CHEN Siyi, SHENG Lisong, SUN Rong. Applications of “Target Fishing” Technique in Pharmacological Research of Traditional Chinese Medicine[J]. Chinese Journal of Pharmacovigilance, 2024, 21(12): 1321-1328.

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https://zgywjj.magtechjournal.com/EN/Y2024/V21/I12/1321

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