Abstract: Objective To establish a method of determining adenosine in Cordyceps Mortierella Mycelia Capsules. Methods We used HPLC to determine adenosine in Cordyceps Mortierella Mycelia Capsules. In this method, HypersilC18 column(4.6 mm×250 mm,5 μm) was used with the mobile phase of KDP buffer solution(0.05 mol·L^-1)-methanol(85:15). The flow rate was 1.0 mL·min^-1, and the wavelength was 260 nm. Results The linear relationship of adenosine within the range of 1.011-50.55g·mL^-1 is fine. The linear equation is A=60901C-2347.7, r=1, and the average recovery μrate is 100.49%(n=6, RSD=1.1%). Conclusion Using HPLC to determine adenosine in Cordyceps Mortierella Mycelia Capsules is not only easy to operate but also without the interference of impurity. It has good precision and the result is reliable. It can be used asa method in the quality control of Cordyceps Mortierella Mycelia Capsules.

Key words: HPLC, determination, Cordyceps Mortierella Mycelia Capsules, adenosine